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Product Name:
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3993
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Mycobacteriaceae, Mycobacterium
Species: Mycobacterium xenopi
Strain: 3993
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Additional Information:
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M. xenopi is an acid-fast, Gram-positive, non-motile, slow-growing non-tuberculous Mycobacterium (NTM) commonly isolated from contaminated water sources.2,3 It is associated with respiratory and pulmonary diseases, with symptoms similar to those of tuberculosis infections, as well as antibiotic resistance, particular in immunocompromised humans.4,5,6 Although M. xenopi was first isolated from an African clawed frog (Xenopus laevis), veterinary diseases associated with M. xenopi are rare, with one report of systemic infection in a bird.7,8
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-44271 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Middlebrook 7H9 broth with ADC enrichment or equivalent
Middlebrook 7H10 agar with OADC enrichment or Lowenstein Jensen agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 6 weeks.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Ordway, D., Personal Communication.
2. Kling, K., et al. “A Cluster of Six Respiratory Cultures Positive for Mycobacterium xenopi – Clinical Characteristics and Genomic Characterization.” J. Clin. Tuberc. Other Mycobact. Dis. 33 (2023): 100397. PubMed: 37727871.
3. Bullin, C. H., E. I. Tanner and C. H. Collins. “Isolation of Mycobacterium xenopei from Water Taps.” J. Hyg. (Lond.) 68 (1970): 97-100. PubMed: 5266590.
4. Varadi, R. G. and T. K. Marras. “Pulmonary Mycobacterium xenopi Infection in Non-HIV-Infected Patients: A Systematic Review.” Int. J. Tuberc. Lung Dis. 13 (2009): 1210-1218. PubMed: 19793424.
5. Bluth, M. H., et al. “Mycobacterium xenopi: Evidence for Increased Rate of Clinical Isolation.” Int. J. Biomed. Sci. 5 (2009): 96-100. PubMed: 23675124.
6. Daley, C. L., et al. “Treatment of Nontuberculous Mycobacterial Pulmonary Disease: An Official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline.” Clin. Infect. Dis. 71 (2020): e1-e36. PubMed: 32628747.
7. Schwabacher, H., et al. “A Strain of Mycobacterium Isolated from Skin Lesions of a Cold-Blooded Animal, Xenopus laevis, and Its Relation to Atypical Acid-Fast Bacilli Occurring in Man.” J. Hyg. (Lond.) 57 (1959): 57-67. PubMed: 13641655.
8. St.-Jean, G., et al. “Mycobacterium xenopi Systemic Infection in a Domestic Fiery-Shouldered Conure Bird (Pyrrhura egregia).” JMM Case Rep. 5 (2018): e005158. PubMed: 30275961.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium xenopi, Strain 3993, NR-44271.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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