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Product Name:
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4529
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Mycobacteriaceae, Mycobacterium
Species: Mycobacterium abscessus (also referred to as Mycobacteroides abscessus)1,2,3 [NR-44273 was deposited to BEI Resources as Mycobacterium xenopi, however, whole genome sequencing performed at BEI Resources resulted in reclassification to Mycobacterium abscessus.]
Strain: 4529
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Additional Information:
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M. abscessus is an acid-fast, Gram-positive, non-motile, non pigmenting, rod-shaped, rapidly growing mycobacterium.5,6 It is highly resistant to a number of antimicrobials, as well as commonly used disinfectants, particularly chlorine.5,6,7 M. abscessus is associated with chronic pneumonia in patients with chronic lung disease and with soft-tissue and post-surgical infections in both community and healthcare settings. This organism has been isolated from human, animal and environmental sources, including soil, bioaerosols and water.7 M. abscessus is subspeciated into M. abscessus subsp. abscessus, M. abscessus subsp. bolletii and M. abscessus subsp. massiliense based on the functionality of an inducible erythromycin methylase (erm) gene, with M. abscessus subsp. massiliense lacking a functional erm.8,9
Reclassification of M. abscessus to the novel genera Mycobacteroides has been proposed following a comprehensive phylogenomic analysis of the genus Mycobacterium, and is currently under debate.1,2,3 This analysis identified 51 highly specific molecular signatures, in the form of conserved signature indels and conserved signature proteins, unique to the Abscessus-Chelonae clade.1
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-44273 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze thaw cycles should be avoided.
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Growth Conditions:
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Media:
Middlebrook 7H9 broth with Middlebrook ADC enrichment or equivalent
Middlebrook 7H10 agar with OADC enrichment or Lowenstein Jensen agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 3 to 7 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Gupta, R. S., B. Lo and J. Son. “Phylogenomics and Comparative Genomic Studies Robustly Support Division of the Genus Mycobacterium into an Emended Genus Mycobacterium and Four Novel Genera.” Front. Microbiol. 9 (2018): 67. PubMed: 29497402.
2. Nouioui, I., et al. “Genome-Based Taxonomic Classification of the Phylum Actinobacteria.” Front. Microbiol. 9 (2018): 2007. PubMed: 30186281.
3. Gupta, R. S. “Commentary: Genome-Based Taxonomic Classification of the Phylum Actinobacteria.” Front. Microbiol. 10 (2019): 206. PubMed: 30853945.
4. Ordway, D., Personal Communication.
5. Adékambi, T., et al. “rpoB Gene Sequence-Based Characterization of Emerging Non-Tuberculous Mycobacteria with Descriptions of Mycobacterium bolletii sp. nov., Mycobacterium phocaicum sp. nov. and Mycobacterium aubagnense sp. nov.” Int. J. Syst. Evol. Microbiol. 56 (2006): 133-143. PubMed: 16403878.
6. Adékambi, T. and M. Drancourt. “Dissection of Phylogenetic Relationships Among 19 Rapidly Growing Mycobacterium Species by 16S rRNA, hsp65, sodA, recA and rpoB Gene Sequencing.” Int. J. Syst. Evol. Microbiol. 54 (2004): 2095-2105. PubMed: 15545441.
7. Brown-Elliott, B. A. and R. J. Wallace, Jr. “Clinical and Taxonomic Status of Pathogenic Nonpigmented or Late-Pigmenting Rapidly Growing Mycobacteria.” Clin. Microbiol. Rev. 15 (2002): 716-746. PubMed: 12364376.
8. Nessar, R., et al. “Mycobacterium abscessus: A New Antibiotic Nightmare.” J. Antimicrob. Chemother. 67 (2012): 810-818. PubMed: 22290346.
9. Griffith, D. E. “Mycobacterium abscessus subsp abscessus Lung Disease: ‘Trouble Ahead, Trouble Behind…’.” F1000Prime Rep. 6 (2014): 107. PubMed: 25580261.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium abscessus, Strain 4529, NR-44273."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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