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Product Name:
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12333
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Staphylococcaceae, Staphylococcus
Species: Staphylococcus epidermidis
Strain: 12333
NARSA Catalog Number: NRS7
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Additional Information:
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S. epidermidis is a Gram-positive, cluster-forming, coagulase-negative coccus which is part of the normal flora of the skin and nostrils. Recently, it has become a common cause of hospital-acquired infections, particularly infections on implanted medical devices.3 A number of factors, such as biofilm formation, small colony variants and a reduced susceptibility to a number of antibiotics, contribute to its success as a cause of nosocomial infections.4,5,6,7 Approximately 75 to 90% of hospital isolates are methicillin-resistant S. epidermidis (MRSE) and an increasing number of isolates have reduced susceptibility to vancomycin.3 Similar to S. aureus, methicillin resistance is conferred by the mecA gene, whereas the reduced susceptibility to vancomycin is due to cell wall
alterations including altered cross-linking and thickening of the wall.3,5,8,9 It is believed that S. epidermidis can serve as a reservoir for antibiotic resistant genes and other genomic islands for S. aureus which can acquire the genes through uni-directional horizontal gene transfer.3
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-45861 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Brain Heart Infusion broth or Tryptic Soy broth or equivalent
Brain Heart Infusion agar or Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. NARSA, NRS7
2. Tenover, F. C., et al. “Characterization of Staphylococci with Reduced Susceptibilities to
Vancomycin and Other Glycopeptides.” J. Clin. Microbiol. 36 (1998): 1020-1027. PubMed: 9542929.
3. Otto, M. “Staphylococcus epidermidis –The ‘Accidental’ Pathogen.” Nat. Rev. Microbiol. 7 (2009): 555-567. PubMed: 19609257.
4. Gazzola, S. and P. S. Cocconcelli. “Vancomycin Heteroresistance and Biofilm Formation in Staphylococcus epidermidis from Food.” Microbiology 154 (2008): 3224-3231. PubMed: 18832327.
5. Qin, Z., et al. “Formation and Properties of In Vitro Biofilms of ica-Negative Staphylococcus epidermidis Clinical Isolates.” J. Med. Microbiol. 56 (2007): 83-93. PubMed: 17172522.
6. Wu, M., et al. “Vancomycin and Daptomycin Pharmacodynamics Differ against a Site-Directed Staphylococcus epidermidisMutant Displaying the Small-Colony-Variant Phenotype.” Antimicrob. Agents Chemother. 53 (2009): 3992-3995. PubMed: 19564372.
7. Al Laham, N., et al. “Augmented Expression of Polysaccharide Intercellular Adhesin in a
Defined Staphylococcus epidermidisMutant with the Small-Colony-Variant Phenotype.” J. Bacteriol. 189 (2007): 4494-4501. PubMed: 17449620.
8. Srinivasan, A., J. D. Dick and T. M. Perl. “Vancomycin Resistance in Staphylococci.” Clin. Microbiol. Rev. 15 (2002): 430-438. PubMed: 12097250.
9. Sanyal, D. and D. Greenwood. “An Electronmicroscope Study of Glycopeptide Antibiotic-Resistant Strains of Staphylococcus epidermidis.” J. Med. Microbiol. 39 (1993): 204-210. PubMed: 8366519.
10. von Eiff, C., et al. “Bloodstream Infections Caused by Small-Colony Variants of Coagulase-Negative Staphylococci Following Pacemaker Implantation.” Clin. Infect. Dis. 29 (1999): 932-934. PubMed: 10589914.
11. Sujatha, S. and I. Praharaj. “Glycopeptide Resistance in Gram-Positive Cocci: A Review.” Interdiscip. Perspect. Infect. Dis. 2012 (2012): 781679. PubMed: 22778729.
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Citation:
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Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution through BEI Resources, NIAID, NIH: Staphylococcus epidermidis, Strain 12333, NR-45861."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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