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Product Name:
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Staphylococcus hominis subsp. novobiosepticus, Strain NRS122
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Staphylococcaceae, Staphylococcus
Species: Staphylococcus hominis subsp. novobiosepticus [NR-45927
was deposited to BEI Resources as Staphylococcus epidermidis, however,
digital DNA-DNA hybridization (dDDH) testing performed at BEI Resources resulted in reclassification to Staphylococcus hominis (S. hominis) subsp. novobiosepticus]
Strain: NRS122
NARSA Catalog Number: NRS122
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Additional Information:
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S. hominisis a Gram-positive, non-motile, coagulase-negative staphylococci (CoNS) that is a commensal resident of human skin covering the axillae, head, legs and arms. It is also an opportunistic pathogen in immunocompromised patients and neonates and has been associated as the causal agent of bacteremia, septicemia and endocarditis.2,3,4 S. hominisis subspeciated into S. hominis, subsp. hominis and S. hominis, subsp. novobiosepticus based on the combined characteristics of novobiocin resistance and failure to produce acid aerobically from D-threhalose and N-acetyl-D-glucosamine in S. hominis, subsp novobiosepticus.5 The two subspecies also differ in the presence and type of SCCmec cassette
they carry. The majority of S. hominis, subsp. novobiosepticus isolates have SCCmec cassette components similar to the ones found in S. aureus, whereas only a small subset of S. hominis, subsp. hominis isolates carry a SCCmec cassette and there is a larger diversity of SCCmec components.6
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-45927 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Brain Heart Infusion broth or Tryptic Soy broth or equivalent
Brain Heart Infusion agar or Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. NARSA, NRS122
2. Kloos, W. E. and K. H. Schleifer. "Isolation and Characterization of Staphylococci from Human Skin. II. Descriptions of Four New Species: Staphylococcus warneri, Staphylococcus hominis, Staphylococcus hominis, and Staphylococcus simulans." Int.
J. Syst. Bacteriol. 25 (1975): 62-79.
3. Chaves, F., et al. “Nosocomial Spread of a Staphylococcus hominis subsp novobiosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit.” J. Clin. Microbiol. 43 (2005): 4877-4879. PubMed: 16145165.
4. Mendoza-Olazarán, S., et al. “Microbiological and Molecular Characterization of Staphylococcus hominis Isolates from Blood.” PLoS One 8 (2013): e61161. PubMed: 23585877.
5. Kloos, W. E., et al. “Staphylococcus hominissubsp. novobiosepticus subsp. nov., a Novel Trehalose- and N-Acetyl-D-Glucosamine-Negative, Novobiocin- and Multiple-Antibiotic-Resistant Subspecies Isolated from Human Blood Cultures.” Int. J. Syst. Bacteriol. 48 (1998): 799-812. PubMed: 9734034.
6. Zhang, L., et al. “Multilocus Sequence Typing and
Further Genetic Characterization of the Enigmatic Pathogen, Staphylococcus hominis.” PLoS One 8 (2013): e66496. PubMed: 23776678.
7. Park, B., T. Iwase and G. Y. Liu. “Intranasal Application of S. epidermidis Prevents Colonization by Methicillin-Resistant Staphylococcus aureus in Mice.” PLoS One 6 (2011): e25880. PubMed: 21998712.
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Citation:
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Acknowledgment for publications should read “The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus hominis subsp. novobiosepticus, Strain NRS122, NR-45927.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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