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Product Name:
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P1V44
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Staphylococcaceae, Staphylococcus
Species: Staphylococcus aureus
Strain: P1V44
NARSA Catalog Number: NRS272
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Additional Information:
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S. aureus is a Gram-positive, cluster-forming coccus that normally inhabits human nasal passages, skin and mucus membranes. It is also a human pathogen and causes a variety of pus-forming infections as well as food-poisoning and toxic shock syndrome. In 1961, two years after the introduction of methicillin, a penicillinase-resistant penicillin, S. aureus developed methicillin-resistance due to acquisition of the mecA gene. Subsequently, MRSA infections have become widespread in both hospital and community settings.3 Vancomycin has been the preferred antibiotic of choice for the treatment of MRSA infections.4 However, there have now been MRSA strains isolated that also have reduced susceptibility or resistance to vancomycin.5,6 It is believed that this decreased sensitivity primarily arises through mutations affecting the production of peptidoglycans, resulting in a thickened cell wall and a reduction of vancomycin at its site of action.7 While much rarer, resistance can also occur through the acquisition of the vancomycin resistance gene, vanA, from Enterococcus faecalis.5,7,8
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-46063 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Brain Heart Infusion broth or Tryptic Soy broth or equivalent
Brain Heart Infusion agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. NARSA, NRS272
2. Denis O., et al. “Emergence of Vancomycin-Intermediate Staphylococcus aureus in a Belgian Hospital: Microbiological and Clinical Features.” J. Antimicrob. Chemother. 50 (2002): 383-391. PubMed: 12205063.
3. Deurenberg, R. H. and E. E. Stobberingh. "The Evolution of Staphylococcus aureus." Infect. Genet. Evol. 8 (2008): 747-763. PubMed: 18718557.
4. Hiramatsu, K. "Vancomycin-Resistant Staphylococcus aureus: A New Model of Antibiotic Resistance." Lancet Infect. Dis. 1 (2001): 147-155. PubMed: 11871491.
5. Hiramatsu, K., et al. "Methicillin-Resistant Staphylococcus aureus Clinical Strain with Reduced Vancomycin Susceptibility." J. Antimicrob. Chemother. 40 (1997): 135-136. PubMed: 9249217.
6. Hanaki, H., et al. "Activated Cell-Wall Synthesis is Associated with Vancomycin Resistance in Methicillin-Resistant Staphylococcus aureus Clinical Strains Mu3 and Mu50." J. Antimicrob. Chemother. 42 (1998): 199-209. Pubmed: 9738837.
7. Howden, B. P., et al. "Reduced Vancomycin Susceptibility in Staphylococcus aureus, Including Vancomycin-Intermediate and Heterogeneous Vancomycin-Intermediate Strains: Resistance Mechanisms, Laboratory Detection, and Clinical Implications." Clin. Microbiol. Rev. 23 (2010): 99-139. PubMed: 20065327.
8. Chang, S., et al. "Infection with Vancomycin-Resistant Staphylococcus aureus Containing the vanA Resistance Gene." N. Engl. J. Med. 3 (2003): 1342-1347. PubMed: 12672861.
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Citation:
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Acknowledgment for publications should read "The following reagent was provided by the Network on Antimicrobial Resistance in Staphylococcus aureus (NARSA) for distribution by BEI Resources, NIAID, NIH: Staphylococcus aureus, Strain P1V44, NR-46063."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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