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Product Name:
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St. Vincent
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Anaplasmataceae, Ehrlichia Species: Ehrlichia chaffeensis Strain: St. Vincent
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Additional Information:
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E. chaffeensis is a Gram-negative, obligate intracellular pathogen of eukaryotic cells and belongs to the alpha subdivision of Proteobacteria. It was originally classified in the family Rickettsiaceae, but subsequently reassigned to the family Anaplasmataceae, both families belonging to the order Rickettsiales.3 E. chaffeensis is transmitted to humans by the lone star tick (Amblyomma americanum) and is the causative agent of HME.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Canis familiaris malignant histiocytosis cells infected with E. chaffeensis, strain St. Vincent, in DMEM (50%) with heat-inactivated FBS (45%), supplemented with 5% dimethyl sulfoxide (DMSO). Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-46445 was packaged aseptically in cryovials and is provided frozen on dry ice. The product should be stored at -130°C or colder, preferably in the vapor phase of a liquid nitrogen freezer. If liquid nitrogen storage facilities are not available, frozen cryovials may be stored at -70°C or colder for approximately one week. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Canis familiaris malignant histiocytosis cells (DH82; ATCC® CRL-3590™) Growth Medium: Dulbecco’s Modified Eagle’s Medium containing 4 mM L-glutamine, 4500 mg per L glucose, 1 mM sodium pyruvate, and 1500 mg per L sodium bicarbonate, supplemented with 7% heat-inactivated fetal bovine serum, or equivalent; optionally, the growth medium may also be supplemented with cycloheximide and additional L-glutamine. Infection: Cells should be 50% to 60% confluent Incubation: 6 to 10 days at 37°C and 5% CO2 Cytopathic Effect: Cell enlargement, rounding, detachment, granularity or other toxicity may or may not be observed. It is recommended that replication of E. chaffeensis be confirmed by PCR, IFA or staining of morulae with Giemsa stain).4
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use.Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.
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References:
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1. Rikihisa, Y., Personal Communication. 2. Paddock, C. D., et al. “Isolation and Characterization of Ehrlichia chaffeensis Strains from Patients with Fatal Ehrlichiosis.” J. Clin. Microbiol. 35 (1997): 2496-2502. PubMed: 9316896. 3. Dumler, J. S., et al. “Reorganization of Genera in the Families Rickettsiaceae and Anaplasmataceae in the Order Rickettsiales: Unification of Some Species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, Descriptions of Six New Species Combinations and Designation of Ehrlichia equi and 'HGE agent' as Subjective Synonyms of Ehrlichia phagocytophila.” Int. J. Syst. Evol. Microbiol. 51 (2001): 2145-2165. PubMed: 11760958. 4. Chen, S.-M., et al. “Cultivation of Ehrlichia chaffeensis in Mouse Embryo, Vero, BGM, and L929 Cells and Study of Ehrlichia–Induced Cytopathic Effect and Plaque Formation.” Infect. Immun. 63 (1995): 647-655. PubMed: 7822034.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ehrlichia chaffeensis, Strain St. Vincent, NR-46445."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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