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NR-46512  Mycobacterium tuberculosis subsp. tuberculosis, H37Rv, Transcription Factor Overexpression Mutant Collection
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis subsp. tuberculosis
Designations: H37Rv, Transcription Factor Overexpression Mutant Collection
Biosafety Level: 3
Availability Status: Discontinued (Please See Comments)
Store at: -60°C or colder
Contributor: D Sherman
Comments:
At this time you may only order individual mutants. Please refer to the attached spreadsheet for individual TFOE mutant information and availability. If you want to order an unavailable mutant please include its item number in the order comments upon checkout.

Mycobacterium tuberculosis (M. tuberculosis) subsp. tuberculosis, strain H37Rv was transformed with N- or C-terminally epitope-tagged expression vectors to produce a collection of transcription factor overexpression (TFOE) mutants.

An anhydrotetracycline (ATc)-inducible episomal vector containing a Gateway® recombination (Invitrogen™) cassette was modified to contain an in-frame N- or C-terminal FLAG epitope tag. A transcription factor-of-interest was selected from a Gateway® entry clone library and recombined into this vector to create epitope-tagged expression vectors (plasmid EXpression N-terminal Flag Tag: pEXNF or plasmid EXpression C-terminal Flag Tag: pEXCF). In the event that the desired transcription factor was not included in the entry clone library, the ORF was sub-cloned from the H37Rv genome using gene-specific oligonucleotides containing Gateway® recombination sequences at the 5' ends. Fidelity of all clones was confirmed by sequencing. Expression of all transcription factors was induced with 100 ng ATc per mL of culture.

Further details relating to applications and the construction of this collection can be found in Nature 499 (2013): 178-183. PubMed: 23823726 or in the supplementary information.

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis subsp. tuberculosis, Strain H37Rv, Transcription Factor Overexpression Mutant Collection, NR-46512."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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