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Product Name:
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FI-01069T (Deposited as Mycobacterium chimaera, strain FI-01069T)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Mycobacteriaceae, Mycobacterium
Species: Mycobacterium intracellulare subsp. chimaera (Previously referred to as Mycobacterium chimaera, this species has reclassified and the designation on the vial label refers to the old nomenclature.)1,2
Strain: FI-01069T (also referred to as DSM 44623T and CIP 107892T)3
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Additional Information:
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M. intracellulare subsp. chimaera is an acid-fast, rod-shaped species of slow growing nontuberculous mycobacteria classified within the Mycobacterium avium complex (MAC).3,5 It is distinguished by a unique mycolic acid pattern obtained by high pressure liquid chromatography (HPLC), which presents two clusters of peaks instead of the three presented by all other MAC organisms.3 M. intracellulare subsp. chimaera has been isolated from human pulmonary samples, rats and environmental samples including water and soil.3,6,7,8
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Material Provided:
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Each vial contains approximately 0.7 mL of bacterial culture in Middlebrook 7H9 broth with ADC enrichment with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-49072 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Middlebrook 7H9 broth with ADC enrichment or equivalent
Middlebrook 7H10 agar with OADC enrichment or Lowenstein-Jensen agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use; then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 2 to 6 weeks.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Nouioui, I., et al. “Genome-Based Taxonomic Classification of the Phylum Actinobacteria.” Front. Microbiol. 9 (2018): 2007. PubMed: 30186281.
2. Oren, A. and G. M. Garrity. “List of New Names and New Combinations Previously Effectively, but not Validly, Published.” Int. J. Syst. Evol. Microbiol. 68 (2018): 3379-3393. PubMed: 30351261.
3. Tortoli, E., et al. “Proposal to Elevate the Genetic Variant MAC-A, Included in the Mycobacterium avium Complex, to Species Rank as Mycobacterium chimaera sp. nov.” J. Syst. Evol. Microbiol. 54 (2004): 1277-1285. PubMed: 15280303.
4. Tortoli, E., Personal Communication.
5. Matos, S., I. Portugal and J. Perdagão. “The Mycobacterium avium Complex: Genomics, Disease, and Beyond.” Microorganisms 13 (2025): 2329. PubMed: 41156789.
6. Honda, J. R., et al. “Environmental Nontuberculous Mycobacteria in the Hawaiian Islands.” PLoS Negl. Trop. Dis. 10 (2016): e0005068. PubMed: 27780201.
7. Durnez, L., et al. “First Detection of Mycobacteria in African Rodents and Insectivores, Using Stratified Pool Screening.” Appl. Environ. Microbiol. 74 (2008): 768-773. PubMed: 18065608.
8. Wallace, R. J., Jr., et al. “Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease.” J. Clin. Microbiol. 51 (2013): 1747-1752. PubMed: 23536397.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium intracellulare subsp. chimaera, Strain FI-01069T (Deposited as Mycobacterium chimaera, Strain FI 01069T), NR-49072.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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