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NR-49807  Escherichia coli, K-12, Strain IM30B
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Designations: K-12, Strain IM30B
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: I Monk, T Stinear, T Foster
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Escherichia coli (E. coli) K-12, strain IM30B contains the hsdM (methylase) and hsdS (specificity) genes from Staphylococcus aureus (S. aureus) MRSA252 clonal complex 30 (CC). This insertion mutant was produced in E. coli K-12, strain DC10B via recombination-mediated genetic engineering (recombineering).

E. coli K-12, strain DC10B is a universal host for constructing plasmids for introduction into staphylococci and was deposited as Δdcm. The lack of methylation allows plasmid DNA to bypass a conserved type IV restriction-modification (RM) barrier in staphylococci which has been identified as a major barrier to transformation with foreign DNA. E. coli K-12, strain IM30B was deposited as resistant to streptomycin.

Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth containing 25 µg/mL streptomycin supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Escherichia coli K-12, Strain IM30B, NR-49807."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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Escherichia coli K-12, Strain DC10B
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Escherichia coli K-12, Strain IM08B
NR-49808
Escherichia coli K-12, Strain IM93B
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