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Product Name:
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AMD (Deposited as Gardnerella vaginalis, Strain AMD)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Bifidobacteriaceae, Gardnerella
Species: Gardnerella leopoldii (Previously referred to as Gardnerella vaginalis, this strain has been reclassified as Gardnerella leopoldii, and the species designation on the vial label refers to the old nomenclature.)1,2
Strain: AMD
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Additional Information:
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G. leopoldii is a facultatively anaerobic bacterium commonly found in vaginal microbiota. It is often described as Gram-variable but has a thin, Gram-positive cell wall.5 Although G. leopoldii is commonly found in healthy individuals, it is one of the predominant organisms of the vaginal cavity in women with bacterial vaginosis.6,7
The G. vaginalis species was recently emended, and three new species within the genus were defined: G. leopoldii, G. swidsinskii, and G. piotii.1,2 Previously referred to as Gardnerella vaginalis genome species 5, G. leopoldii is distinguishable from other Gardnerella spp. based on average nucleotide identity (ANI) values, DNA-DNA hybridization (DDH) and MALDI-TOF profiles.1
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in NYC III broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-50514 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
NYC III broth or equivalent
Chocolate agar or Casman’s medium base with 5% Rabbit Blood or Tryptic Soy agar with 5% sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Anaerobic or aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 to 4 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Hill, J. E., A. Y. K. Albert and VOGUE Research Group. “Resolution and Cooccurrence Patterns of Gardnerella leopoldii, G. swidsinskii, G. piotii, and G. vaginalis within the Vaginal Microbiome.” Infect. Immun. 87 (2019): e00532-19. PubMed: 31527125.
2. Vaneechoutte, M., et al. “Emended Description of Gardnerella vaginalis and Description of Gardnerella leopoldii sp. nov., Gardnerella piotii sp. nov. and Gardnerella swidsinskii sp. nov., with Delineation of 13 Genomic Species within the Genus Gardnerella.” Int. J. Syst. Evol. Microbiol. 69 (2019): 679-687. PubMed: 30648938.
3. Jefferson, K. K. and G. A. Buck, Personal Communication.
4. Harwich, M. D., Jr., et al. “Drawing the Line Between Commensal and Pathogenic Gardnerella vaginalis Through Genome Analysis and Virulence Studies.” BMC Genomics 11 (2010): 375. PubMed: 20540756.
5. Harper, J. J. and G. H. G. Davis. “Cell Wall Analysis of Gardnerella vaginalis (Haemophilus vaginalis).” Int. J. Syst. Bacteriol. 32 (1982): 48-50.
6. Aroutcheva, A. A., et. al. “Gardnerella vaginalis Isolated from Patients with Bacterial Vaginosis and from Patients with Healthy Vaginal Ecosystems.” Clin. Infect. Dis. 33 (2001): 1022-1027. PubMed: 11528575.
7. Yeoman, C. J., et al. “Comparative Genomics of Gardnerella vaginalis Strains Reveals Substantial Differences in Metabolic and Virulence Potential.” PLoS One 5 (2010): e12411. PubMed: 20865041.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Gardnerella leopoldii, Strain AMD (Deposited as Gardnerella vaginalis, Strain AMD), NR-50514.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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