Disclaimer: You are now leaving www.beiresources.org and are going to a website that is not operated by BEI Resources. We are not responsible for the content or availability of linked sites.
ABOUT THIRD PARTY LINKS ON OUR SITE: BEI Resources offers links to other third party websites that may be of interest to our website visitors. The links provided in our website are provided solely for your convenience and may assist you in locating other useful information on the Internet. When you click on these links, you will leave the BEI Resources website and will be redirected to another site. These sites are not under the control of BEI Resources. BEI Resources is not responsible for the content of linked third party websites. We are neither an agent for these third parties nor do we endorse or guarantee their products. We make no representation or warranty regarding the accuracy of the information contained in the linked sites. We suggest that you always verify the information obtained from linked websites before acting upon this information. Please read third party privacy and security policies closely as these may be different than BEI Resources policies. If you have any questions or concerns about the products and services offered on linked third party websites, please contact the third party directly.
Are you sure you would like to be notified when the item is available?
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Burkholderia pseudomallei (B. pseudomallei), strain Bp82 is an attenuated strain of B. pseudomallei, strain 1026b. Strain Bp82 was attenuated via a partial deletion of the purM gene, resulting in adenine and thiamine auxotrophy.
B. pseudomallei, strain Bp82 is an adenine auxotroph that was engineered through deletion of a portion of the purM gene, which encodes phosphoribosyl formylglycinamide cycloligase. The product of the reaction catalyzed by this enzyme, aminoimidazole ribotide, is a precursor of de novo adenine and thiamine biosynthesis. The partial deletion of the purM gene was confirmed through PCR and sequencing. Strain Bp82 was tested and found to be avirulent in mouse and hamster models. The parent strain, 1026b, was originally isolated in 1993 from a blood culture of a female rice farmer with diabetes mellitus at Sappasithiprasong hospital in Ubon, Ratchathani, Thailand, and is available from BEI Resources as NR-4074.The complete genome sequence of B. pseudomallei, strain 1026b has been determined by two sequencing centers (GenBank: CP002833.1, CP002834.1 and CP004379.1, CP004380.1).
B. pseudomallei, strain Bp82 (ΔpurM) is excluded from Select Agent status. Please refer to the Select Agent Exclusions at the National Select Agent Registry website for more information.
Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
When applying for permits and forms please:
Do NOT reference BEI Resources or ATCC Catalog Numbers in the Material Description fields found on the permits and/or forms.
Make sure your name and address on the permit applications and/or forms are exactly as they appear on your BEI Resources registration.
Information about permits is provided as a courtesy to BEI Resources customers. While we use reasonable efforts to include accurate and up-to-date information on this page, we make no warranties or representation as to its accuracy.
For more information on the necessary compliance requirements associated with the biological materials provided by BEI Resources, please select this link: Compliance Requirements.