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NR-51280  Burkholderia pseudomallei, Bp82 (ΔpurM)
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Burkholderia pseudomallei
Designations: Bp82 (ΔpurM)
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: HP Schweizer
Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Burkholderia pseudomallei (B. pseudomallei), strain Bp82 is an attenuated strain of B. pseudomallei, strain 1026b. Strain Bp82 was attenuated via a partial deletion of the purM gene, resulting in adenine and thiamine auxotrophy.

B. pseudomallei, strain Bp82 is an adenine auxotroph that was engineered through deletion of a portion of the purM gene, which encodes phosphoribosyl formylglycinamide cycloligase. The product of the reaction catalyzed by this enzyme, aminoimidazole ribotide, is a precursor of de novo adenine and thiamine biosynthesis. The partial deletion of the purM gene was confirmed through PCR and sequencing. Strain Bp82 was tested and found to be avirulent in mouse and hamster models. The parent strain, 1026b, was originally isolated in 1993 from a blood culture of a female rice farmer with diabetes mellitus at Sappasithiprasong hospital in Ubon, Ratchathani, Thailand, and is available from BEI Resources as NR-4074.The complete genome sequence of B. pseudomallei, strain 1026b has been determined by two sequencing centers (GenBank: CP002833.1, CP002834.1 and CP004379.1, CP004380.1).

B. pseudomallei, strain Bp82 (ΔpurM) is excluded from Select Agent status. Please refer to the Select Agent Exclusions at the National Select Agent Registry website for more information.

Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.

Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Burkholderia pseudomallei, Strain Bp82 (ΔpurM), NR-51280.”
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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