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NR-51673 Borrelia recurrentis, PAbn
(Bacteria)

Price: All BEI Resources products are provided
at no cost to registered researchers.

Organism:

Borrelia recurrentis

Designations:

PAbn

Biosafety Level:

Availability Status:

In Stock

Store at:

-60°C or colder

Contributor:

Dr. Volker Fingerle, National Reference Center (NRZ) for Borrelia, Bavarian Health and Food Safety Authority, Oberschleissheim, Germany

Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Borrelia recurrentis (B. recurrentis), strain PAbn was isolated in Germany in 2015 from the blood of a human with louse-borne relapsing fever who originated in Ethiopia and migrated to Germany.

B. recurrentis, strain PAbn is reported to be multi-locus sequence type (MLST) ST-669. The complete genome of B. recurrentis, strain PAbn has been sequenced (GenBank: GCF_042466535.1).

Material Provided:

Each vial contains approximately 0.5 mL of bacterial culture in Revised Barbour-Stoenner-Kelly broth supplemented with 15% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.

Citations:

Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Borrelia recurrentis, Strain PAbn, NR-51673.”

Restrictions On Use:

This material is distributed for internal research, non-commercial purposes only. This material, its product or its derivatives may not be distributed to third parties. Except as performed under a U.S. Government contract, individuals contemplating commercial use of the material, its products or its derivatives must contact the contributor to determine if a license is required. U.S. Government contractors may need a license before first commercial sale.

Product Name:

PAbn

Manufacturer:

BEI Resources

Taxonomy:

Bacteria Classification: Spirochaetaceae, Borrelia

Species: Borrelia recurrentis

Strain: PAbn

Product Description:

Original Source: Borrelia recurrentis (B. recurrentis), strain PAbn was isolated in Germany in 2015 from the blood of a human with louse-borne relapsing fever who originated in Ethiopia and migrated to Germany.^1,2

Comments: B. recurrentis, strain PAbn is reported to be multi‑locus sequence type (MLST) ST-669.² The complete genome of B. recurrentis, strain PAbn has been sequenced (GenBank: GCF_042466535.1).²

Additional Information:

B. recurrentis is a large, loosely coiled, motile spirochete transmitted by a single known vector, Pediculus humanus (body louse).^2,3,4 It is the causative agent of louse-borne relapsing fever (LBRF), an epidemic disease with a distinctive relapsing phenomenon endemic to the Horn of Africa that is re-emerging in Europe, primarily along refugee migration routes.^2,3 B. recurrentis has the most simple genome of all Borrelia spp., composed of one linear chromosome, seven linear plasmids and 990 protein-coding genes.^2,3 Genomic analysis indicates that B. recurrentis and B. duttoni, which causes tick-borne relapsing fever, are nearly identical and cannot be differentiated by sequencing of the 16S ribosomal RNA gene.^2,3 Approximately 30 genes or gene families are missing or damaged in B. recurrentis, including the RecA and RadA proteins involved in DNA double-strand break repair, suggesting that B. recurrentis is a decaying strain of B. duttonii.^2,3,4

Material Provided:

Each vial contains approximately 0.5 mL of bacterial culture in Revised Barbour-Stoenner-Kelly broth supplemented with 15% glycerol.

Note: If homogeneity is required for your intended use, please purify prior to initiating work.

Packing/Storage:

NR-51673 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.

Growth Conditions:

Media:

Revised Barbour-Stoenner-Kelly broth or equivalent (Appendix I)

Note: Medium should be prepared fresh before each use.

Incubation:

Temperature: 32°C to 34°C

Atmosphere: Aerobic with 5% CO₂ or microaerophilic

Propagation:

Note: It is recommended that NR-51673 be cultured in 24-well plates until growth is established from the frozen vial. The culture may be initiated in both atmospheres to determine the optimal growth condition for this strain.

1. Place the frozen vial in a 35°C to 37°C water bath and thaw for approximately 2 to 3 minutes. Immerse the vial just enough to cover the frozen material. Do not agitate the vial. Do not leave the vial in the water bath after it is thawed.

2. Immediately after thawing, aseptically transfer the contents of the vial to 2 wells of a 24-well plate containing 1.5 mL fresh Revised Barbour-Stoenner-Kelly medium per well.

3. Incubate the plate at 32°C to 34°C. Do not shake culture during growth. It may take up to 21 days for the culture to establish from the frozen state.

Note: NR-51673 should be subcultured during the log phase of growth, as viability of the culture may decrease quickly.

Maintenance:

1. Monitor growth of the culture by live/dead staining every 3 to 6 days. When the culture has reached the log phase, transfer approximately 2 mL into to a T-25 tissue culture flask containing 8 mL fresh Revised Barbour-Stoenner-Kelly medium.

2. Incubate the plate at 32°C to 34°C.

3. Transfer the culture every 3 to 21 days as described in Maintenance steps 1 and 2. The transfer interval will depend on the size of the inoculum and the quality of the medium. This should be determined by performing live/dead staining every 3 to 6 days. Do not allow the culture to overgrow. Viability of the culture may be affected soon after reaching peak density.

Disclaimers:

You are authorized to use this product for research use only. It is not intended for human use.

Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC^® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC^® nor the U.S. Government warrants that such information has been confirmed to be accurate.

This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC^® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC^®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.

References:

1. Fingerle, V., Personal Communication.

2. Marosevic, D., et al. “First Insights in the Variability of Borrelia recurrentis Genomes.” PLoS Negl. Trop. Dis. 11 (2017): e0005865. PubMed: 28902847.

3. Warrell, D. A. “Louse-Borne Relapsing Fever (Borrelia recurrentis Infection).” Epidemiol. Infect. 147 (2019): e106. PubMed: 30869050.

4. Lescot, M., et al. “The Genome of Borrelia recurrentis, the Agent of Louse-Borne Relapsing Fever, is a Degraded Subset of Tick-Borne Borrelia duttonii.” PLoS Genet. 4 (2008): e1000185. PubMed: 18787695.

Citation:

Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Borrelia recurrentis, Strain PAbn, NR-51673.”

Biosafety Level:

2 Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.

Dynamic Product Sheet

Certificate of Analysis by Lot

Other

NR-51673 Appendix I – Revised Barbour-Stoenner-Kelly (BSK) Broth

All compliant version documentation requests will be addressed within two business days of receipt.

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Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

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Product Highlights for NR-51673

Product Sheet Information for NR-51673 Borrelia recurrentis, PAbn, For research use only. Not for use in humans.

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