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Product Name:
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MRSN Diversity Panel
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Additional Information:
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P. aeruginosa is a Gram-negative, aerobic, rod-shaped bacterium with unipolar motility that thrives in many diverse environments including soil, water and certain eukaryotic hosts. It is a key emerging opportunistic pathogen in animals, including humans and plants. While it rarely infects healthy individuals, P. aeruginosa causes severe acute and chronic nosocomial infections in immunocompromised or catheterized patients, especially in patients with cystic fibrosis, burns, cancer or HIV.3,4,5 Infections of this type are often highly antibiotic resistant, difficult to eradicate, and often lead to death. The ability of P. aeruginosa to survive on minimal nutritional requirements, tolerate a variety of physical conditions, and rapidly develop resistance during the course of therapy, has allowed it to persist in both community and hospital settings.5,6
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Material Provided:
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Each panel contains one vial of each P. aeruginosa MRSN isolate for a total of 100 vials. Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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Each isolate was packaged aseptically in cryovials and assembled into one 100-section freezer box. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended.
Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or Brain Heart Infusion broth or Nutrient broth or equivalent
Tryptic Soy agar with 5% defibrinated sheep blood or Brain Heart Infusion agar or Nutrient agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. McGann, P., Personal Communication.
2. Tsao, Y.-F., et al. “Phage Morons Play an Important Role in Pseudomonas aeruginosa Phenotypes.” J. Bacteriol. 200 (2018): e00189-18. PubMed: 30150232.
3. Silva Filho, L. V., et al. “Pseudomonas aeruginosa Infection in Patients with Cystic Fibrosis: Scientific Evidence Regarding Clinical Impact, Diagnosis, and Treatment.” J. Bras. Pneumol. 39 (2013): 495-512. PubMed: 24068273.
4. Dettman, J. R., et al. “Evolutionary Genomics of Epidemic and Nonepidemic Strains of Pseudomonas aeruginosa.” Proc. Natl. Acad. Sci. USA 110 (2013): 21065-21070. PubMed: 24324153.
5. Morita, Y., J. Tomida and Y. Kawamura. “Responses of Pseudomonas aeruginosa to Antimicrobials.” Front. Microbiol. 4 (2014): 422. PubMed: 24409175.
6. Lister, P. D., D. J. Wolter and N. D. Hanson. “Antibacterial-Resistant Pseudomonas aeruginosa: Clinical Impact and Complex Regulation of Chromosomally Encoded Resistance Mechanisms.” Clin. Microbiol. Rev. 22 (2009): 582-610. PubMed: 19822890.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Pseudomonas aeruginosa MRSN Diversity Panel, NR-51829, provided by the Multidrug-Resistant Organism Repository and Surveillance Network (MRSN) at the Walter Reed Army Institute of Research (WRAIR).”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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