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Product Name:
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MRSN Diversity Panel
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Material Provided:
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Each panel contains one vial of each K. pneumoniae MRSN isolate, for a total of 100 vials. Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol. Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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Each isolate was packaged aseptically in cryovials and assembled into one 100-section freezer box. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media: Nutrient broth or Tryptic Soy broth or equivalent Nutrient agar or Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent Incubation: Temperature: 37°C Atmosphere: Aerobic Propagation: 1. Keep vial frozen until ready for use, then thaw. 2. Transfer the entire thawed aliquot into a single tube of broth. 3. Use several drops of the suspension to inoculate an agar slant and/or plate. 4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
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Patents or other restrictions:
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This material may be subject to third party patent rights.
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References:
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1. McGann, P., Personal Communication. 2. Martin, M. J., W. Stribling, A. C. Ong, R. Maybank, Y. I. Kwak, J. A. Rosado-Mendez, L. N. Preston, K. F. Lane, M. Julius, A. R. Jones, M. Hinkle, P. E. Waterman, E. P. Lesho, F. Lebreton, J. W. Bennett and P. T. Mc Gann. “A Panel of Diverse Klebsiella pneumoniae Clinical Isolates for Research and Development.” Microb. Genom. 9 (2023). PubMed: 37141116. 3. Lascols, C., et al. “Increasing Prevalence and Dissemination of NDM-1 Metallo-β-Lactamase in India: Data from the SMART Study (2009).” J. Antimicrob. Chemother. 66 (2011): 1992-1997. PubMed: 21676902. 4. Ramirez, M. S., et al. “Multidrug-Resistant (MDR) Klebsiella pneumoniae Clinical Isolates: A Zone of High Heterogeneity (HHZ) as a Tool for Epidemiological Studies.” Clin. Microbiol. Infect. 18 (2012): E254-E258. PubMed: 22551038. 5. Podschun, R. and U. Ullmann. “Klebsiella spp. as Nosocomial Pathogens: Epidemiology, Taxonomy, Typing Methods, and Pathogenicity Factors.” Clin. Microbiol. Rev. 11 (1998): 589-603. PubMed: 9767057.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Klebsiella pneumoniae MRSN Diversity Panel, NR-55604, provided by the Multidrug-Resistant Organism Repository and Surveillance Network (MRSN) at the Walter Reed Army Institute of Research (WRAIR), Silver Spring, Maryland, USA.”
In addition, the contributors’ paper should be acknowledged: "Martin, M. J., W. Stribling, A. C. Ong, R. Maybank, Y. I. Kwak, J. A. Rosado-Mendez, L. N. Preston, K. F. Lane, M. Julius, A. R. Jones, M. Hinkle, P. E. Waterman, E. P. Lesho, F. Lebreton, J. W. Bennett and P. T. Mc Gann. “A Panel of Diverse Klebsiella pneumoniae Clinical Isolates for Research and Development.” Microb. Genom. 9 (2023). doi: 10.1099/mgen.0.000967. PubMed: 37141116."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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