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Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
ARP-11365 is a construct consisting of approximately 14 kilobases including the insert, nef from human immunodeficiency Virus 2 (HIV-2), isolate HIV-2A 60415k2 (GenBank: DQ092764). The size of the insert is approximately 11 kilobases. The vector is a truncated form of pBR322 containing the eGFP reporter gene and the gene for ampicillin resistance.
nef alleles from different primate lentiviruses were cloned into an HIV-1 (NL4-3 based) proviral vector designed to co-express nef and eGFP from a single bicistronic RNA. The nef expression is mediated by the wild-type HIV-1 LTR promoter and naturally occurring splice sites. Cells infected with these reporter viruses co-express Nef and eGFP at correlating levels. The effect of Nef on the surface expression of cellular receptors or on apoptosis can be examined directly in virally infected cells.
Note: Target cells can either be infected using the wild-type X4-tropic NL4-3 Env or VSV-G pseudotyped viral particles.
Each vial of ARP-11365 contains approximately 5 µg of dried, purified DNA stabilized in DNAstable®Plus (Biomatrica®). Please refer to the appropriate data sheet for lot-specific information.
Clones 11349 and 11350: Schindler, M., et al. “Down-Modulation of Mature MHC Class II and Up-Regulation of Invariant Chain Cell Surface Expression Are Well Conserved Functions of Human and Simian Immunodeficiency Virus nef Alleles.” J. Virol. 77 (2003): 10548-10556. PubMed: 12970439. Schindler, M., J. Munch and F. Kirchhoff. “HIV-1 Inhibits DNA Damage Triggered Apoptosis by a Nef-Independent Mechanism.” J. Virol. 79 (2005): 5489-5498. PubMed: 15827163. Clones 11351-11380: Schindler, M., et al. “ Nef-Mediated Suppression of T Cell Activation Was Lost in a Lentiviral Lineage that Gave Rise to HIV-1.” Cell 125 (2006): 1055-1067. PubMed: 16777597. For more information on this series of IMCs, also see:
Kirchhoff, F. et al. “Nef Proteins from Simian Immunodeficiency Virus-Infected Chimpanzees Interact with p21-Activated Kinase 2 and Modulate Cell Surface Expression of Various Human Receptors.” J. Virol. 78 (2004): 6864-6874. PubMed: 15194762 Munch, J. et al. “Nef-Mediated Enhancement of Virion Infectivity and Stimulation of Viral Replication Are Fundamental Properties of Primate Lentiviruses.” J. Virol. 81 (2007): 13852-13864. PubMed: 17928336.
The supplemental data in the following reference has a helpful table including the IMCs in this panel plus others: Heigele, A., et al. “Down-Modulation of CD8alpha-beta Is a Fundamental Activity of Primate Lentiviral Nef Proteins.” J. Virol. 86 (2012): 36-48. PubMed: 22013062.
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