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NR-15182   Vector pCAGGS Containing SARS Coronavirus Nonstructural Protein 1
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at no cost to registered researchers.
Description: Vector pCAGGS Containing SARS Coronavirus Nonstructural Protein 1
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: P Kuhn
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The nonstructural protein 1 (nsp1) coding sequence from the TOR-2 strain of the severe acute respiratory syndrome-related coronavirus (SARS-CoV) was subcloned into a modified pCAGGS mammalian expression vector. The construct was engineered to encode a C-terminal fragment containing linker sequences, a hemagglutinin (HA) tag, a tobacco etch virus (TEV) protease cleavage site, and a 23 amino acid sequence that is site-specifically biotinylated by the Escherichia coli (E.coli) biotin ligase BirA. The resulting plasmid was named pCC438. NR-15182 was produced in E. coli TOP10 cells (Invitrogen™) and extracted using a QIAGEN® Plasmid Plus Maxi Kit.

Nsp1 and associated cellular proteins can be efficiently purified from HEK293/ACE2 cells co-transfected with NR-15182 and a plasmid encoding BirA using streptavidin paramagnetic beads.

NR-15182 has been qualified for use in bacterial transformations.

Each vial contains approximately 100 µL of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pCAGGS Containing SARS Coronavirus Nonstructural Protein 1, NR-15182."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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