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NR-19270   SARS Coronavirus, Tor2, Complete Gateway® Clone Set, Recombinant in Escherichia coli
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: SARS Coronavirus, Tor2, Complete Gateway® Clone Set, Recombinant in Escherichia coli
Organism: SARS coronavirus
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

SARS Coronavirus, Tor2, Gateway® clones were designed for features based on the annotation from the GenBank entry AY274119 and the corresponding RefSeq entry for NC_004718. The sequences from both entries are identical but there are annotation differences. The clones were designed from the annotated ORFs from AY274119 and from the annotated protein coding regions of NC_004718. The clone set consists of twenty-seven clones that were constructed in vector pDONR 221 (Invitrogen). ATG start codons were added to the forward primer sequences when required and stop codon sequence was trimmed from the reverse primer sequences. Each clone has been sequenced using a combination of end sequencing and primer walking to determine each base at an average of 2-fold coverage. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Each well of the 96-well plate contains approximately 40 µL of Escherichia coli culture [strain DH10B-T1, or strain Stbl for clones NC_828851, NC_828869(a) and NC_828869(b)] in Luria Bertani (LB) Broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Sheet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: SARS Coronavirus, Tor2, Complete Gateway ® Clone Set, Recombinant in Escherichia coli, NR-19270."
Additional Information: Invitrogen Limited Use Label License
Master Clone List
Publications Citing
this Reagent:
Häuser, R., et al. "A Second-Generation Protein-Protein Interaction Network of Helicobacter pylori." Mol. Cell. Proteomics 13 (2014): 1318-1329. PubMed: 24627523.
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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