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NR-19274   Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42
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at no cost to registered researchers.
Description: Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.

NR-19274 consists of Plates 1-42 (BEI Resources NR-19637 through NR-19678) of the Mycobacterium tuberculosis (M. tuberculosis) Gateway® clone set. Detailed information is available on the Product Information Sheet and Certificate of Analysis for each individual plate.

The M. tuberculosis Gateway® clone set (Plates 1-42) contains 3724 sequence validated clones (3294 M. tuberculosis, strain H37Rv clones supplemented with 430 unique open reading frames (ORF) from M. tuberculosis, strain CDC1551) cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR 221 with an ATG start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Information related to the use of Gateway® Clones can be obtained from Invitrogen™. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR™ 221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Every inoculated well of each 96-well plate contains approximately 40 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Note: Production in the 96-well format has a potential for cross-contamination. Individual mutants should be checked by the recipient prior to use.

Additional information is available at the Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis Gateway® Clone Set, Recombinant in Escherichia coli, Plates 1-42, NR-19274."
Additional Information: Invitrogen Limited Use Label License
Master Clone List
Publications Citing
this Reagent:
Häuser, R., et al. "A Second-Generation Protein-Protein Interaction Network of Helicobacter pylori." Mol. Cell. Proteomics 13 (2014): 1318-1329. PubMed: 24627523.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

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