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NR-19435  Influenza A virus, H1N1pdm09 Expression Clone Set, Recombinant in Escherichia coli
(Clones)
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at no cost to registered researchers.
Description: H1N1pdm09 Expression Clone Set, Recombinant in Escherichia coli
Organism: Influenza A virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The H1N1pdm09 Expression Clone Set (also 2009 H1N1 Expression Clone Set) contains Influenza A (H1N1)pdm09 open reading frames from two clinical isolates A/New York/1682/2009 (H1N1)pdm09 (CY039901 to CY039908) and A/New York/1669/2009 (H1N1)pdm09 (CY039893 to CY039900) cloned in Escherichia coli (E. coli) DH10B cells. The clone set consists of thirty clones that were constructed in either pIVE-LIC-His_cHalo, or the Promega vectors pFC20A or pFC14A. The full annotated coding sequence (CDS) for each genomic segment has been cloned (stop codons removed) and the sequence verified. The hemagglutinin CDS was truncated to remove the 3' transmembrane domain and the tailing sequence and the neuraminidase CDS was spliced to remove the 5' transmembrane domain but retain the 5' eighteen base pair leader sequence.

Information related to the use of the expression vector can be obtained from Promega.

Each well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B) in Luria Bertani (LB) broth containing 100 µg/mL ampicillin supplemented with 15% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H1N1pdm09 Expression Clone Set, Recombinant in Escherichia coli, NR-19435."
Additional Information: Master Clone List
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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