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NR-19439   Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14
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at no cost to registered researchers.
Description: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14
Organism: Escherichia coli
Biosafety Level: 2
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue.  Please allow ample time for distribution lots to be made available.

NR-19439 consists of Plates 1-14 (BEI Resources NR-19790-NR-19803) of the Entertoxigenic Escherichia coli (E. coli) expression clone set. Information for each plate is available on the Master Clone List.

The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of 14 plates which contain approximately 917 sequence validated clones from E. coli strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed in vector pMCSG7 (a pET21 derivative; for routine HTP purification). The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Plate orientation and viability were confirmed for each plate of the set.

Every inoculated well of each 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 100 µg/mL ampicillin supplemented with 15% glycerol.

Clone plates are replicated using a BioMek® FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plates 1-14, NR-19439."
Additional Information: Invitrogen Limited Use Label License
Master Clone List
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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