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NR-19457   Rickettsia prowazekii Gateway® Clone Set, Recombinant in Escherichia coli, Plate 9
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Description: Rickettsia prowazekii Gateway® Clone Set, Recombinant in Escherichia coli, Plate 9
Organism: Rickettsia prowazekii
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Rickettsia prowazekii (R. prowazekii) Gateway® clone set consists of approximately 748 sequence validated clones from R. prowazekii, strain Madrid E cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR™221 (Invitrogen™) with an ATG start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Information related to the use of Gateway® Clones can be obtained from Invitrogen™. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR™221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Plate orientation and viability were confirmed for NR-19457.

Each inoculated well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Clone plates are replicated using a BioMek® FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Rickettsia prowazekii Gateway® Clone Set, Recombinant in Escherichia coli, Plate 9, NR-19457."
Additional Information: Limited Use Label License
NR-19457 JCVI Plate 09 Map
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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