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NR-19686   Vibrio cholerae Gateway® Clone Set, Recombinant in Escherichia coli, Plate 8
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Vibrio cholerae Gateway® Clone Set, Recombinant in Escherichia coli, Plate 8
Organism: Vibrio cholerae
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Vibrio cholerae (V. cholerae) Gateway® clone set consists of 46 plates which contain 3813 sequence validated clones from V. cholerae, strain El Tor N16961 cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR™221 with a native start codon and stop codon. The library was independently cloned and sequence verified by the Harvard Institute of Proteomics.

Information related to the use of Gateway® Clones can be obtained from Invitrogen™. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR™221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Each inoculated well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Note:  Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vibrio cholerae Gateway® Clone Set, Recombinant in Escherichia coli, Plate 8, NR-19686."
Additional Information: Invitrogen Limited Use License
NR-19686 JCVI Plate 08 Map
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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