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NR-19792   Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 3
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 3
Organism: Escherichia coli
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of approximately 900 sequence validated clones from Escherichia coli (E. coli) strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed, using ligation independent cloning, in vector pMCSG7 (a pET21 derivative that contains an N-terminal 6xHis tag; for routine HTP purification). The sequence was validated by full length sequencing of each clone (using 5' and 3' primers; TACTTCCAATCC AATGCG and TTATCC ACTTCCAATG, respectively) with greater than 1X coverage and a mutation rate of less than 0.2%.

Each inoculated well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 100 µg/mL ampicillin supplemented with 15% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Information Sheet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 3, NR-19792."
Additional Information: Limited Use Label License
NR-19792 JCVI Plate 03 Map
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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