Contact Us   Having technical issues or questions regarding this product?
Please Contact Us.
BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-19803   Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 14
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 14
Organism: Escherichia coli
Biosafety Level: 2
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of 14 plates which contain 917 sequence validated clones from Escherichia coli (E. coli) strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed in vector pMCSG7 (a pET21 derivative; for routine HTP purification). The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Each inoculated well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 100 µg/mL ampicillin supplemented with 15% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Information Sheet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 14, NR-19803."
Additional Information: NR-19803 JCVI Plate 14 Map
Limited Use Label License
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Return to Top

Notices and Disclaimers

BEI Resources products are intended for laboratory research purposes only. They are not intended for use in humans.

While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this site, BEI Resources makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. BEI Resources does not warrant that such information has been confirmed to be accurate.

Back to My Search