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NR-36034  Cavia porcellus, Guinea Pig Expression Clone IL-1β, Recombinant in Escherichia coli
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Guinea Pig Expression Clone IL-1β, Recombinant in Escherichia coli
Organism: Cavia porcellus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: DN McMurray
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-36034 is an expression clone containing the mature peptide region of IL-1β (GenBank: AF119622) from Cavia porcellus (guinea pig). The complete IL-1β gene was cloned into vector pET-30a(+) via BamHI and HindIII insertion sites and transformed into Escherichia coli (E. coli) NovaBlue competent cells. After the presence of the insert was verified, the plasmid DNA was isolated and transformed into E. coli, strain Rosetta 2(DE3) for protein expression. The pET-30a(+) vector contains a T7 promoter, genes to allow kanamycin and chloramphenicol resistance, an N-terminal His-tag for purification, and the lacI gene which is used for enhanced protein expression via IPTG induction.

Each vial contains approximately 0.5 mL of E. coli, strain Rosetta 2(DE3) in Luria Bertani (LB) broth containing 15 µg/mL kanamycin and 34 µg/mL chloramphenicol supplemented with 10% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Guinea Pig Expression Clone IL-1β, Recombinant in Escherichia coli, NR-36034."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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