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NR-36204  Cavia porcellus, Guinea Pig Expression Clone CD8α/pcDNA3.1 Hygro(+)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Guinea Pig Expression Clone CD8α/pcDNA3.1 Hygro(+)
Organism: Cavia porcellus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: RG Rank
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-36204 is a full-length expression clone containing the CD8α gene (GenBank: NM_001172876) from guinea pig (Cavia porcellus). The CD8α gene was cloned into vector pcDNA3.1/hygro(+) via BamHI and EcoRV insertion sites, transformed into MAX Efficiency®Escherichia coli (E. coli) DH5α™ competent cells (Invitrogen™), and extracted using a QIAGEN® Plasmid Mega Kit.

Note: Sequencing results for NR-36204 revealed that most regions of the vector match the depositor's information, including the ampicillin resistance gene, the hygromycin resistance gene under control of the SV40 promoter, polyadenylation signals, and the pUC and f1 origins of replication. However, the native cytomegalovirus (CMV) promoter has been replaced by a ubiquitin (UbC) promoter upstream of CD8α this replacement also resulted in the loss of the T7 promoter. The resulting size of the plasmid is 6.735 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-36204.

Each vial of NR-36204 contains approximately 1 µg of plasmid DNA in TE buffer. The concentration is shown on the Certificate of Analysis.

Note: The vial labels for NR-36204 incorrectly state that the plasmid DNA is provided as an E. coli glycerol stock. NR-36204 is purified plasmid DNA that was extracted from bacteria prior to vialing.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Guinea Pig Expression Clone CD8α/pcDNA3.1 Hygro(+), NR-36204."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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