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NR-36206  Cavia porcellus, Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+)
(Clones)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+)
Organism: Cavia porcellus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: RG Rank
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-36206 is a full-length expression clone containing the CD8β gene (GenBank: AY303774.1) from guinea pig (Cavia porcellus). The CD8β gene was cloned into pcDNA3.1/hygro(+) via BamHI and EcoRV insertion sites, transformed into MAX Efficiency®Escherichia coli (E. coli) DH5α™ competent cells (Invitrogen™), and extracted using a QIAGEN® Plasmid Mega Kit.

Note: Sequencing results for NR-36206 revealed that most regions of the vector match the depositor's information, including the ampicillin resistance gene, the hygromycin resistance gene under control of the SV40 promoter, polyadenylation signals, and the pUC and f1 origins of replication. However, the native cytomegalovirus (CMV) promoter has been replaced by a ubiquitin (UbC) promoter upstream of CD8β; this replacement also resulted in the loss of the T7 promoter. The hygromycin resistance gene contains an in-frame 21-base-pair deletion which removes seven amino acids near the C-terminal end of the protein (not near the active site). The resulting size of the plasmid is 6.540 kilobases. The plasmid map and the complete plasmid sequence are provided on the Certificate of Analysis for NR-36206.

Each vial of NR-36206 contains approximately 1 µg of plasmid DNA in TE buffer.

Note: The vial labels for NR-36206 incorrectly state that the plasmid DNA is provided as an E. coli glycerol stock. NR-36206 is purified plasmid DNA that was extracted from bacteria prior to vialing.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Guinea Pig Expression Clone CD8β/pcDNA3.1 Hygro(+), NR-36206."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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