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Product Name:
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Talaromyces marneffei, 03-130
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Manufacturer:
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BEI Resources
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Taxonomy:
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Classification: Trichocomaceae; Talaromyces
Species: Talaromyces marneffei (previously classified as Penicillium marneffei)
Strain/Isolate: 03-130 (also referred to as 11CN-03-130)1
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Additional Information:
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T. marneffei is a thermally dimorphic, saprophytic, fungus endemic to Southeast Asia and China.1,3 It is the causative agent of talaromycosis, an invasive, opportunistic infection and leading cause of death in HIV-infected individuals, with an increasing rate of diagnosis in HIV-negative patients with other forms of immunosuppression.4,5,6 Clinical manifestations in HIV-positive patients often include skin lesions, fever, cough, weight loss, anemia, diarrhea, hepatomegaly, lymphadenopathy and splenomegaly.6,7
Bamboo rats and humans are the most prevalent animal hosts of T. marneffei, with transmission to humans suspected to occur through inhalation of airborne conidia from soil or another environmental source common between the hosts.7,8
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Material Provided:
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Each vial contains approximately 0.5 mL of yeast cells in 20% glycerol.
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Packing/Storage:
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NR-60072 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long term storage the product should be stored -130°C or colder, preferably in the vapor phase of a liquid nitrogen freezer.
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Thawing and Growth:
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Media:
Potato Dextrose agar or Czapek Yeast Extract agar or Malt Extract agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use; thaw rapidly in a water bath at 30°C. Typically, this takes less than 5 minutes.
2. Immediately after thawing, inoculate an agar plate with approximately 50 µL of thawed culture.
3. Incubate the plate at 37°C for 2 to 8 days.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Cuomo, C. A., et al. “Complete Genome Sequences for Two Talaromyces marneffei Clinical Isolates from Northern and Southern Vietnam.” Microbiol. Resour. Announc. 9 (2020): e01367-19. PubMed: 31919177.
2. Le, T., Personal Communication.
3. Wang, F., R. Han and S. Chen. “An Overlooked and Underrated Endemic Mycosis-Talaromycosis and the Pathogenic Fungus Talaromyces marneffei.” Clin. Microbiol. Rev. 36 (2023): e0005122. PubMed: 36648228.
4. Le, T., et al. “A Trial of Itraconazole or Amphotericin B for HIV-Associated Talaromycosis.” N. Engl. J. Med. 376 (2017): 2329-2340. PubMed: 28614691.
5. Supparatpinyo, K., et al. “Disseminated Penicillium marneffei Infection in Southeast Asia.” Lancet 344 (1994): 110-113. PubMed: 7912350.
6. Chan, J. F. W., et al. “Talaromyces (Penicillium) marneffei Infection in Non-HIV-Infected Patients.” Emerg. Microbes Infect. 5 (2016): e19. PubMed: 26956447.
7. Vanittanakom, N., et al. “Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects.” Clin. Microbiol. Rev. 19 (2006): 95-110. PubMed: 16418525.
8. Wong, S. Y. N. and K. F. Wong. “Penicillium marneffei Infection in AIDS.” Patholog. Res. Int. 2011 (2011): 764293. PubMed: 21331327.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Talaromyces marneffei, Strain 03-130, NR-60072.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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