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NR-52309   Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene RBD with C-Terminal Hexa-Histidine Tag
(Plasmid/Vectors)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene RBD with C-Terminal Hexa-Histidine Tag
Organism: SARS-Related Coronavirus 2
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: F Krammer, F Amanat, S Strohmeier
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The vector for the receptor binding domain (RBD) of the spike (S) glycoprotein gene from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenBank: MN908947) was designed by fusing the N-terminal S protein signal sequence to the spike RBD (amino acids 319 to 541) with a C-terminal hexa-histidine tag. The sequence was codon optimized for mammalian expression and subcloned into the pCAGGS mammalian expression vector under the AG promoter. NR-52309 contains the beta-lactamase gene, TEM-116, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The resulting size of the plasmid is approximately 5490 base pairs. The complete plasmid sequence is provided on the BEI Resources webpage. The plasmid was produced in Escherichia coli and extracted.

Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/

The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.

Each vial of lot 70033695 contains 2.0 µg of plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. Each vial of lot 70034636 contains 1.4 µg of plasmid DNA in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Citations: Acknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Vector pCAGGS Containing the SARS-Related Coronavirus 2, Wuhan-Hu-1 Spike Glycoprotein Gene RBD with C-Terminal Hexa-Histidine Tag, NR-52309.”
Restrictions On Use:
Registrants interested in commercializing this product must contact Mount Sinai for a license (Frenz, Christopher christopher.frenz@mssm.edu).
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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Spike Glycoprotein Receptor Binding Domain (RBD) from SARS-Related Coronavirus 2, Wuhan-Hu-1, Recombinant from HEK293 Cells
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