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NR-9577  Bacillus anthracis, Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli
Organism: Bacillus anthracis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-9577 is a recombinant form of the Bacillus anthracis (B. anthracis) collagen-like protein BclA (locus_tag: BA_1222), a major component of the exosporium. The amino acid sequence includes 1) an N-terminal hexa-histidine tag, 2) a thrombin cleavage site and 3) the complete coding sequence (amino acid residues 1 to 400) of BclA from the Sterne strain (GenPept: AAT53453). The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-9577 has a theoretical molecular weight of approximately 39 kilodaltons. The structure of BclA has been solved (PDB: 1WCK; 2R6Q).

Each vial contains approximately 80 to 120 µg of NR-9577 in PBS containing 2 M urea.

NR-9577 reacts with rabbit polyclonal antibody to B. anthracis BclA (BEI Resources NR-9578) as shown by western blot analysis.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Collagen-like Protein BclA (Locus_Tag: BA_1222) with N-terminal Histidine Tag, Recombinant from Escherichia coli, NR-9577."
Publications Citing
this Reagent:
Goldman, E. R., et al. "Negative Tail Fusions Can Improve Ruggedness of Single Domain Antibodies." Protein Expr. Purif. 95 (2014): 226-232. PubMed: 24440507.

Walper, S. A., et al. "Selection and Characterization of Single Domain Antibodies Specific for Bacillus anthracis Spore Proteins." Antibodies 2 (2013): 152-167.

Walper, S. A., et al. "Thermostable Single Domain Antibody-Maltose Binding Protein Fusion for Bacillus anthracis Spore Protein BclA Detection." Anal. Biochem. 447 (2014): 64-73. PubMed: 24184358
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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