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NR-15495   Vaccinia Virus, Western Reserve, Recombinant Expressing Lassa Virus, Josiah Nucleoprotein
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Description: Vaccinia Virus, Western Reserve, Recombinant Expressing Lassa Virus, Josiah Nucleoprotein
Organism: Lassa virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: MJ Buchmeier
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A cDNA clone containing the entire ORF encoding the nucleoprotein from segment S of Lassa virus (LASV), Josiah was inserted into the pRB21 transfer vector, bringing it under the control of a synthetic vaccinia virus (VACV) early/late promoter (PSYN). Recombinant VACV was made by transfecting the transfer plasmid into CV-1 cells infected with the VACV strain vRB12.

LASV is an Arenavirus (Arenaviridae, Arenavirus) which is the etiologic agent of Lassa fever.

Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (BSC-40, ATCC ® CRL-2761™) infected with vaccinia virus, rVACV-LASV NP.

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Recombinant Expressing Lassa Virus, Josiah Nucleoprotein, NR-15495."
Publications Citing
this Reagent:
Botten, J., et al. "Coverage of Related Pathogenic Species by Multivalent and Cross-Protective Vaccine Design: Arenaviruses as a Model System." Microbiol. Molec. Biol. Rev. 74 (2010): 157-170. PubMed: 20508245.

Kotturi, M. F., et al. "Polyfunctional CD4+ T Cell Responses to a Set of Pathogenic Arenaviruses Provide Broad Population Coverage." Immunome Res. 6 (2010): 4. PubMed: 20478058.

Ziegler, C. (2013). "4 Pathogenic Old World." Viral Hemorrhagic Fevers: 233.
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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