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NR-3685  Influenza A virus, Kilbourne F125: A/Victoria/3/1975 (HA) x A/equine/Prague/1/1956 (NA) x A/Puerto Rico/8/1934 (H3N7), Reassortant X-48R
(Viruses)
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Description: Kilbourne F125: A/Victoria/3/1975 (HA) x A/equine/Prague/1/1956 (NA) x A/Puerto Rico/8/1934 (H3N7), Reassortant X-48R
Organism: Influenza A virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: NIAID/NIH
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-3685 was created by crossing X-47 (Kilbourne F123: NR-3663) with influenza A/equine/Prague/1/1956 (H1N7). X-47 is A/Victoria/3/1975 (HA, NA) x A/Puerto Rico/8/1934 (H3N2). Nucleotide sequencing at BEI Resources of a portion of the matrix (M) gene (RNA 7) from NR-3685 indicates that the M gene is derived from A/equine/Prague/1/1956 (H7N7). Since the complete genotype of X-47 has been determined, it is possible to infer that the polymerase basic subunit 2 (PB2) gene (RNA 1), the polymerase acidic subunit (PA) gene (RNA 3), and the nonstructural protein (NS) gene (RNA 8) are derived from either A/equine/Prague/1/1956 (H7N7) or A/Puerto Rico/8/1934 (H1N1) while the polymerase basic subunit 1 (PB1) gene (RNA 2) and the nucleoprotein (NP) gene (RNA 6) are derived from either A/Victoria/3/1975 (H3N2) or A/equine/Prague/1/1956 (H7N7).

Each vial contains approximately 1 mL of cell lysate and supernatant from Madin-Darby Canine Kidney (MDCK) cells (ATCC ® CCL-34™) infected with reassortant influenza A virus, A/Victoria/3/1975 (HA) x A/equine/Prague/1/1956 (NA) x A/Puerto Rico/8/1934 (H3N7).

Kilbourne Archive Homepage
Kilbourne/New York Medical College Archive of Influenza Virus Reassortants and Mutants
Kilbourne F125 information
Virus
Download (Word Docs):Detailed Description
File Number:F125
Virus Name:
Virus Attributes:Type A Virus. reassortant. animal virus - human virus hybrids. antigenic (HA/NA) hybrid. human HA-specific antigenic hybrid
 
 
Antisera
AntiserumImmunizing VirusLocationAntiserum Attributes
3651X-48RB29antigenic (HA/NA) hybrid. HA Specific,
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Kilbourne F125: A/Victoria/3/1975 (HA) x A/equine/Prague/1/1956 (NA) x A/Puerto Rico/8/1934 (H3N7), Reassortant X-48R, NR-3685."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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