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NR-41567  Eastern equine encephalitis virus, FL93-939
(Viruses)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: FL93-939
Organism: Eastern equine encephalitis virus
Biosafety Level: 3
Availability Status: In Stock
Store at: -60°C or colder
Contributor: SC Weaver
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Eastern equine encepahilitis virus (EEEV), FL93-939 was isolated in Vero cells from a pool of Culiseta melanura mosquitoes collected in Florida in 1993, and passaged once in suckling mouse brains to generate RNA. An infectious cDNA clone of the viral genome was constructed and rescued by electroporation of in vitro transcribed RNA. The complete genome sequence of EEEV, FL93-939 has been determined (GenBank: EF151502).

EEEV is a zoonotic virus transmitted by mosquitoes and originating in birds. The virus can cause severe disease in horses, pigs, dogs, and some bird species. EEEV was first isolated in 1933 from the brains of infected horses in Virginia and New Jersey. Four lineages of EEEV have been defined based on antigenicity and geographic distribution. North American (lineage I) viruses are the most virulent in horses and humans. Human infections are usually asymptomatic, but some progress to severe encephalitis with a high fatality rate. Since horses and humans do not serve as amplifying hosts, infected birds are critical in perpetuating the viral life cycle.

Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero 76; ATCC ® CCL-1587™) infected with eastern equine encephalitis virus (EEEV), FL93-939.

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Eastern Equine Encephalitis Virus, FL93-939, NR-41567."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.
  • Select Agents require facility registration for specific agents with CDC and completion of APHIS/CDC Form 2. For information about toxin amounts excluded from CDC Select Agent regulations, click here.

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