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Product Name:
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DENV-2/US/BID-V680/1994
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Flaviviridae, Flavivirus
Species: Dengue virus type 2
Strain/Isolate: DENV-2/US/BID-V680/1994
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Additional Information:
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Dengue virus causes the most common vector-borne viral disease of humans, with over 50 million cases in tropical and subtropical regions each year.3 The disease is now endemic in over 110 countries in the world, with Southeast Asia and the Western Pacific being the most seriously affected. Dengue disease is caused by one of four closely related, but antigenically distinct serotypes (designated DEN-1 to -4).3 Infections produce a spectrum of clinical illness ranging from a nonspecific viral syndrome to severe and fatal hemorrhagic disease.4,5 Humans are the major host of dengue virus, with Aedes mosquitoes as the principal vectors.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Aedes albopictus mosquito larval epithelial cells (clone C6/36; ATCC® CRL-1660™) infected with dengue virus type 2, DENV-2/US/BID-V680/1994.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-44219 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Aedes albopictus clone C6/36 cells (ATCC® CRL-1660™)
Growth Medium: Eagle’s Minimum Essential Medium containing Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate and 1.5 g/L of sodium bicarbonate supplemented with 2% fetal bovine serum, or equivalent
Infection: Cells should be 70% to 80% confluent
Incubation: 7 days at 28°C and 5% CO2
Cytopathic Effect: Inconsistent; syncytia formation and cell detachment may or may not be observed.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Munoz-Jordan, J. L., Personal Communication.
2. Henn, M. R., et al. Broad Institute of MIT and Harvard, Cambridge, MA, USA. Direct Submission.
3. Holmes, E. C. and S. S. Twiddy. “The Origin, Emergence and Evolutionary Genetics of Dengue Virus.” Infect. Genet. Evol. 3 (2003): 19-28. PubMed: 12797969.
4. Malavige, G. N., et al. “Dengue Viral Infections.” Postgrad. Med. J. 80 (2004): 588-601. PubMed: 15466994.
5. Kao, C. L., et al. “Laboratory Diagnosis of Dengue Virus Infection: Current and Future Perspectives in Clinical Diagnosis and Public Health.” J. Microbiol. Immunol. Infect. 38 (2005): 5-16. PubMed: 15692621.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Dengue Virus Type 2, DENV-2/US/BID-V680/1994, NR-44219."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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