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Product Name:
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A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Orthomyxoviridae, Alphainfluenzavirus
Species: Influenza A virus
Reassortant: A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8)
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Additional Information:
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The deduced amino acid sequence of the hemagglutinin gene of influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) indicates avian-like receptor binding preference and low pathogenic potential in poultry.2
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Material Provided:
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Each vial contains approximately 1 mL of pooled allantoic fluid from specific pathogen free (SPF) embryonated chicken eggs infected with reassortant influenza A virus, A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8).
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-49439 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Safety Precautions:
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Biosafety Level 2 facilities with enhanced practices and procedures are recommended for research and production activities utilizing reassortant viruses carrying the internal genes from A/Puerto Rico/8/1934 and the surface genes from avian-origin influenza viruses isolated from human infections. ATCC® treats these viruses as Biosafety Level 3 material for purposes of packaging for safe shipment.
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Growth Conditions:
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Host: 9- to 11-day-old SPF embryonated chicken eggs
Infection: Embryonated chicken eggs must be candled for viability prior to inoculation
Incubation: 2 days at 35°C in a humidified chamber
Effect: Hemagglutination activity using chicken or turkey red blood cells and allantoic fluid from infected embryonated chicken eggs
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
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Patents or other restrictions:
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The Wisconsin Alumni Research Foundation (“WARF”) owns intellectual property including patents, such as US 8,715,940, which may read on use of NR-49439. Any commercial use of this material may require a license from WARF. Please contact WARF at licensing@warf.org or (608) 263-2500 for further information.
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References:
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1. Wohlbold, T. J., A. Hirsch, and F. Krammer. “An H10N8 Influenza Virus Vaccine Strain and Mouse Challenge Model Based on the Human Isolate A/Jiangxi-Donghu/346/13.” Vaccine 33 (2015): 1102-1106. PubMed: 25604801.
2. Chen, H., et al. “Clinical and Epidemiological Characteristics of a Fatal Case of Avian Influenza A H10N8 Virus Infection: A Descriptive Study.” Lancet 383 (2014): 714-721. PubMed: 24507376.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A Virus, A/Jiangxi-Donghu/346/2013 (HA, NA) x A/Puerto Rico/8/1934 (H10N8), NR-49439."
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Biosafety Level:
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2+
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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