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NR-727  Vaccinia virus, Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells
(Viruses)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells
Organism: Vaccinia virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -70°C or colder
Contributor: LS Wyatt, B Moss
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The early passage history of MVA is complex. The complete genomic sequence of MVA has been determined (GenBank: U94848).

MVA is a highly attenuated strain of vaccinia virus and does not appear to replicate in most mammalian cells. Some experts feel MVA can be handled safely by trained personnel without the need for vaccination.

NR-727 was prepared by inoculation of hamster kidney cells (BHK-21) with VACV, MVA. The virus was purified from a crude cell lysate and supernatant by centrifugation through a 36% sucrose cushion.

A sucrose-purified preparation of BHK-21 cells for use as a control is available as BEI Resources NR-794.

Each vial contains approximately 1 mL of NR-727 in TE (pH 7.4).

Additional information and tools are available at ViPR (Virus Pathogen Resource).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Modified Vaccinia Ankara (MVA), Purified From BHK-21 Cells, NR-727."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-794
Mesocricetus auratus BHK-21 Cell Lysate, Mock Infected, Purified
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