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Product Name:
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TRVL-11573
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Arenaviridae, Mammarenavirus
Species: Tacaribe virus (TCRV) (Mammarenavirus tacaribeense)
Strain/Isolate: TRVL-11573
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Additional Information:
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TCRV is a new world arenavirus which, although highly homologous to Junin virus and lethal to mice, has low pathogenic potential for humans. TCRV shares a similar genomic organization with all other arenaviruses, displaying a bipartite, ambisense, single-stranded RNA genome.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from African green monkey kidney cells [Vero (E6); ATCC® CRL-1586™] infected with TCRV, TRVL-11573.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-10175 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Vero (E6) cells (ATCC® CRL-1586™)
Growth Medium: Eagle’s Minimum Essential Medium supplemented with 2% irradiated fetal bovine serum, or equivalent
Infection: Cells should be 60-80% confluent (not 100% confluent)
Incubation: 6 to 10 days at 37°C and 5% CO2
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Downs, W. G., et al. “Tacaribe Virus, a New Agent Isolated from Artibeus Bats and Mosquitoes in Trinidad, West Indies.” Am. J. Trop. Med. Hyg. 12 (1963): 640- 646.
2. Franze-Fernández, M. T., et al. “Molecular Structure and Early Events in the Replication of Tacaribe Arenavirus S RNA.” Virus Res. 7 (1987): 309-324. PubMed: 3617928.
3. Iapalucci, S., N. López and M. T. Franze-Fernández. “The 3' End Termini of the Tacaribe Arenavirus Subgenomic RNAs.” Virology 182 (1991): 269-278. PubMed: 2024465.
4. Iapalucci, S., et al. “Tacaribe Virus L Gene Encodes a Protein of 2210 Amino Acid Residues.” Virology 170 (1989): 40-47. PubMed: 2718387.
5. Iapalucci, S., et al. “The 5' Region of Tacaribe Virus L RNA Encodes a Protein with a Potential Metal Binding Domain.” Virology 173 (1989): 357-361. PubMed: 2510403.
6. Pedras-Vasconcelos, J. A., et al. “Immunotherapy with CpG Oligonucleotides and Antibodies to TNF-alpha Rescues Neonatal Mice from Lethal Arenavirus-Induced Meningoencephalitis.” J. Immunol. 180 (2008): 8231- 8240. PubMed: 18523289.
7. Charrel, R. N., X. de Lamballerie and S. Emonet. “Phylogeny of the Genus Arenavirus.” Curr. Opin. Microbiol. 11 (2008): 362-368. PubMed: 18602020.
8. Bowen, M. D., C. J. Peters, and S. T. Nichol. “The Phylogeny of New World (Tacaribe Complex) Arenaviruses.” Virology 219 (1996): 285-290. PubMed: 8623541.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Tacaribe Virus, TRVL-11573, NR-10175."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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