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NR-28634  Influenza A virus, A/Hong Kong/1/1968-2 Mouse-Adapted 21-2 (H3N2)
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Description: A/Hong Kong/1/1968-2 Mouse-Adapted 21-2 (H3N2)
Organism: Influenza A virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: EG Brown
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Influenza A virus, A/Hong Kong/1/1968-2 mouse-adapted 21-2 (H3N2) was derived from a virus isolated from a human in Hong Kong in 1968.

Sequence information is available for influenza A virus, A/Hong Kong/1-2-MA21-2/1968 (H3N2) at the Influenza Research Database. Note that although NR-28634 was deposited to BEI Resources as A/Hong Kong/1/1968-2 mouse-adapted 21-2 (H3N2), nucleotide sequence obtained from the same source material by the NIAID Influenza Genome Sequencing Consortium was deposited to NCBI and IRD as A/Hong Kong/1-2-MA21-2/1968 (H3N2).

The prototype strain of the 1968 influenza epidemic in Hong Kong was originally isolated in primary monkey kidney cells by W. K. Chang and sent to H. G. Pereira at the WHO World Influenza Center in London, from whom it was subsequently obtained by the Laboratory Center for Disease Control, Health Canada, Ottawa. The virus was passaged twice in rhesus monkey kidney cells and three times in the allantoic cavity of embryonated chicken eggs before two plaque purifications in Madin-Darby canine kidney (MDCK) cells and re-amplification in embryonated chicken eggs. This virus (available as BEI Resources NR-28620) was subcloned by an additional round of plaque purification in MDCK cells. Subclone 2 was re-amplified again by two egg passages and inoculated intranasally into CD-1 mice. Virus extracts were prepared from lung homogenates after three days. After 21 sequential mouse passages, a clonal isolate was obtained by two plaque purifications in MDCK cells. The mouse-adapted virus was passaged twice in specific pathogen free embryonated chicken eggs before deposit to BEI Resources. Specific mutations in several viral genes are associated with adaptation to the mouse lung and evolution to increased virulence. Other mutations, or combinations of mutations, are unique to certain isolates, and can be used to identify each individual mouse-adapted variant.

Each vial contains approximately 1 mL of pooled allantoic fluid from specific pathogen free (SPF) embryonated chicken eggs infected with influenza A virus, A/Hong Kong/1/1968-2 mouse-adapted 21-2 (H3N2).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A Virus, A/Hong Kong/1/1968-2 Mouse-Adapted 21-2 (H3N2), NR-28634."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Influenza A Virus, A/Hong Kong/1/1968 (H3N2) (mother clone)
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