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NR-3688  Influenza A virus , Kilbourne F68: A/WSN/1933 (H1N1), Wild Type (ts+) Clone
(Viruses)
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at no cost to registered researchers.
Description: Kilbourne F68: A/WSN/1933 (H1N1), Wild Type (ts+) Clone
Organism: Influenza A virus
Biosafety Level: 2
Availability Status: In Stock
Contributor: NIAID/NIH
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Kilbourne F68 is a heat stable (ts+) clone of the WSN strain of influenza A virus derived by three successive rounds of plaque purification on Madin-Darby Bovine Kidney cells propagated at 39.5°C.  The cloned virus was used to generate a series of temperature-sensitive influenza mutants including Kilbourne F66 (ts51), a well characterized matrix gene mutation that causes nuclear retention of the M1 protein.  Kilbourne F66 is availabe as BEI Resources NR-3687.

Sequence information is available for influenza A virus, A/WSN/1933 (H1N1) at the Influenza Research Database.

Removal of contaminating mycoplasma from NR-3688 required six additional virus passages at BEI Resources.

Each vial contains approximately 1 mL of cell lysate and supernatant from Madin-Darby Canine Kidney (MDCK) cells (ATCC ® CCL-34) infected with influenza A virus, A/WSN/1933 (H1N1), wild type (ts+) clone.
Virus
Download (Word Docs):Detailed Description
File Number:F68
Virus Name:WSN ts+ (wt clone) H1N1
Virus Attributes:Type A Virus. A subtype H1N1. mutant. human virus. human HA-specific antigenic hybrid . potentially neuroviolent in mice
 
 
Antisera
AntiserumImmunizing VirusLocationAntiserum Attributes
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Influenza A virus, Kilbourne F68: A/WSN/1933 (H1N1), Wild Type (ts+) Clone, NR-3688."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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