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Product Name:
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21005-2WR (Tissue Culture Adapted) (formerly Parainfluenza Virus 5)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Paramyxoviridae, Orthorubulavirus
Species: Orthorubulavirus 5 (formerly parainfluenza virus 5, simian virus 5)
Strain/Isolate: 21005-2WR
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Additional Information:
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NR-42515 was derived from NIAID catalog number V-322-011-000. It was adapted to growth in tissue culture by three passages in rhesus monkey kidney epithelial (LLC-MK2) cells (ATCC® CCL-7.1™).
Although first described as a contaminant of monkey cell cultures, the true origin and natural host of mammalian rubulavirus 5 is not clear. It is associated with kennel cough in dogs, and is thus often referred to as canine parainfluenza virus in the veterinary literature.3,4 Mammalian rubulavirus 5 is not known to cause disease in humans or other animals.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from rhesus monkey kidney cells infected with tissue culture adapted mammalian rubulavirus 5, 21005-2WR.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-42515 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Macaca mulatta kidney cells (LLC-MK2, ATCC® CCL- 7.1™)
Growth Medium: Dulbecco’s Modified Eagle’s Minimum Essential Medium, or equivalent, supplemented with 4 µg/mL trypsin and 10% fetal bovine serum
Infection: Cells should be 80% confluent
Incubation: 2 to 5 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and sloughing
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Hull, R. N., J. R. Minner and J. W. Smith. “New Viral Agents Recovered from Tissue Cultures of Monkey Kidney Cells. I. Origin and Properties of Cytopathic Agents S.V.1, S.V.2, S.V.3, S.V.4, S.V.5, S.V.6, S.V.11, S.V.12 and S.V.15.” Am. J. Hyg. 63 (1956): 204-215. PubMed: 13302209.
2. Kuhn, J. H., et al. "2022 Taxonomic Update of Phylum Negarnaviricota (Riboviria: Orthornavirae), including the Large Orders Bunyavirales and Mononegavirales." Arch. Virol. 167 (2022): 2857-2906. PubMed: 36437428.
3. McCandlish, I. A., et al. “A Study of Dogs with Kennel Cough.” Vet. Rec. 102 (1978): 293-301. PubMed: 207006.
4. Chen, Z., et al. “Evaluating a Parainfluenza Virus 5-Based Vaccine in a Host with Pre-existing Immunity against Parainfluenza Virus 5.” PLoS One 7 (2012): e50144. PubMed: 23185558.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mammalian Rubulavirus 5, 21005-2WR (Tissue Culture Adapted), NR-42515.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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