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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-43286  Porcine respiratory coronavirus, ISU-1
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: ISU-1
Organism: Porcine respiratory coronavirus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: LJ Saif
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Porcine Respiratory Coronavirus (PRCV), ISU-1 was isolated by Howard Hill in Indiana in 1990 from a nasal swab of a pig with mild or subclinical respiratory infection. It was passaged eight times in swine testicular (ST) cells and plaque purified twice. At least seven additional passages and a third round of plaque purification were performed prior to deposit at BEI Resources.

PRCV was first isolated from pigs in Belgium that were seropositive for porcine transmissible gastroenteritis virus (TGEV) despite lacking any history of clinical TGE, and was subsequently also isolated from pig herds in the United States. PRCV is not an officially recognized alphacoronavirus species. It is actually a naturally occurring deletion mutant of TGEV (now known as alphacoronavirus 1), with altered respiratory tropism and reduced virulence. The complete genome of the ISU-1 strain has been sequenced (GenBank: DQ811787).

NR-43286 was derived from BEI Resources NR-448 by removal of contaminating gram-negative bacteria and mycoplasma. This process required six additional passages in ST cells (ATCC ® CRL-1746™). NR-448 is no longer available.

Each vial contains approximately 1 mL of clarified cell lysate and supernatant from Sus scrofa testicular fibroblasts infected with porcine respiratory coronavirus, ISU-1.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Porcine Respiratory Coronavirus, ISU-1, NR-43286."
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
  • USDA APHIS VS 16-6 or 16-6A permit must be obtained and a copy of the permit must be sent to ATCC in advance of shipment. The Application Form VS 16-3 (Import controlled material import or transport organisms or vectors) must be submitted to USDA APHIS Veterinary Services to obtain the VS 16-6 or 16-6A permit.

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