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Product Name:
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Thottapalayam
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Hantaviridae, Thottimvirus (formerly Bunyaviridae, Hantavirus)
Species: Thottapalayam (TPM) virus
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Additional Information:
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Despite being the first hantavirus isolated, TPM virus remains somewhat of an enigma, in that it was not isolated from a rodent (rather an insectivore) and has been shown to be the most genetically and antigenically distinct of all hantaviruses to date.2,3,4,5,6
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from African green monkey cells (Vero; ATCC® CCL-81™) infected with TPM virus.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-465 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Vero cells (ATCC® CCL-81™)
Growth Medium: Minimum Essential Medium supplemented with 2% irradiated fetal bovine serum, or equivalent (lot-specific details are on the Certificate of Analysis)
Infection: Cells should be 80–90% confluent (not 100% confluent)
Incubation: 6 to 8 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and detachment
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Carey, D. E., et al. “Thottapalayam Virus: A Presumptive Arbovirus Isolated from a Shrew in India.” Indian J. Med. Res. 59 (1971): 1758–1760. PubMed: 5169031.
2. Yadav, P. D., M. J. Vincent, and S. T. Nichol. “Thottapalayam Virus Is Genetically Distant to the Rodent-Borne Hantaviruses, Consistent with Its Isolation from the Asian House Shrew (Suncus murinus).” Virol. J. 4 (2007): 80. PubMed: 17711577.
3. Song, J.-W., L. J. Baek, C. S. Schmaljohn, and R. Yanagihara. “Thottapalayam Virus, a Prototype Shrew-Borne Hantavirus.” Emerg. Infect. Dis. 13 (2007): 980–985.
4. Okamura, M., et al. “Development of Serological Assays for Thottapalayam Virus, an Insectivore-Borne Hantavirus.” Clin. Vaccine Immunol. 14 (2007): 173–181. PubMed: 17182762.
5. Chu, Y. K., et al. “Serological Relationships among Viruses in the Hantavirus Genus, Family Bunyaviridae.” Virology 198 (1994): 196–204. PubMed: 8259655.
6. Xiao, S.-Y., et al. “Phylogenetic Analyses of Virus Isolates in the Genus Hantavirus, Family Bunyaviridae.” Virology 198 (1994): 205–217. PubMed: 8259656.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through the NIH Biodefense and Emerging Infections Research Resources Repository, NIAID, NIH: Thottapalayam Virus, NR-465."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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