| |
|
Product Name:
|
NCH-1
|
|
Manufacturer:
|
BEI Resources
|
|
Taxonomy:
|
Bacteria Classification: Anaplasmataceae, Anaplasma
Species: Anaplasma phagocytophilum
Strain: NCH-1
|
|
Additional Information:
|
A. phagocytophilum is a Gram-negative, obligate intracytoplasmic bacteria that infects bone marrow-derived mammalian cells, predominantly of the myeloid lineage.3 The species was formerly known as Ehrlichia phagocytophila and classified in the family Rickettsiaceae, but subsequently reassigned to the family Anaplasmataceae, both families belonging to the order Rickettsiales.3 A. phagocytophilum is transmitted by Ixodes persulcatus complex ticks and is the causative agent of tick-borne fever of ruminants, equine granulocytic ehrlichiosis, and human granulocytic ehrlichiosis (HGE).3,4
|
|
Material Provided:
|
Each vial contains approximately 1 mL of human promyelocytic leukemia cells (HL-60; ATCC® CCL-240™) infected with A. phagocytophilum, strain NCH-1 containing fetal bovine serum and DMSO (final concentrations are indicated on the Certificate of Analysis for each lot).
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
|
|
Packing/Storage:
|
NR-48807 was packaged aseptically
in cryovials and is provided frozen on dry ice. The
product should be stored at -130°C or colder, preferably in the vapor phase of
a liquid nitrogen freezer. If liquid nitrogen storage facilities are not
available, frozen cryovials may be stored at -70°C or colder for approximately
one week. Freeze-thaw cycles should be
avoided.
|
|
Growth Conditions:
|
Host: HL-60 cells (ATCC® CCL-240™)
Growth Medium: RPMI-1640 medium containing 2 mM L-glutamine, 10 mM HEPES, 4500 mg/L glucose, 1 mM sodium pyruvate and 1500 mg/L sodium bicarbonate, supplemented with 10% fetal bovine serum, or equivalent.
Infection: Cells should be at a dilution of 1 × 105 to 1 × 106 cells/mL.
Incubation: 8 days at 37°C and 5% CO2
Cytopathic Effect: Uninfected HL-60 cells are typically round with smooth borders. Infected cell borders are rough in appearance. It is recommended that replication of A. phagocytophilum be confirmed by indirect fluorescent antibody (IFA) assay.
|
|
Disclaimers:
|
You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
|
1. Carlyon, J., Personal Communication.
2. Kolber, C. P., et al. “Characterization of an Immunoreactive Protein from the Agent of Human Granulocytic Ehrlichiosis” J. Clin. Microbiol. 35 (1997): 1172-1178. PubMed: 9114402.
3. Dumler, J. S., et al. “Reorganization of Genera in the Families Rickettsiaceae and Anaplasmataceae in the Order Rickettsiales: Unification of Some Species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia, and Ehrlichia with Neorickettsia, Descriptions of Six New Species Combinations and Designation of Ehrlichia equi and 'HGE agent' as Subjective Synonyms of Ehrlichia phagocytophila.” Int. J. Syst. Evol. Microbiol. 51 (2001): 2145-2165. PubMed: 11760958.
4. Dumler, J. S., et al. “Human Granulocytic Anaplasmosis and Anaplasma phagocytophilum.” Emeg. Infect. Dis. 11 (2005): 1828-1834. PubMed: 16485466.
|
|
Citation:
|
Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Anaplasma phagocytophilum, Strain NCH-1, NR-48807.”
|
|
Biosafety Level:
|
2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
|