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Product Name:
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TRVL 4675
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Togaviridae, Alphavirus
Species: Mayaro virus
Strain/Isolate: TRVL 4675
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Additional Information:
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MAYV is a New World alphavirus that is the etiologic agent of Mayaro fever, an acute febrile illness sometimes accompanied by severe and persistent arthritis. MAYV was first isolated in Trinidad in 1954, and there have been sporadic outbreaks of Mayaro fever in South America since.3 The enzootic transmission cycle of MAYV is not fully understood, but the occurrence of relatively large outbreaks of Mayaro fever and the competence of Aedes mosquitoes for transmission of MAYV suggest the potential for an urban human-mosquito-human transmission cycle to emerge.4,5
There are two distinct genotypes of MAYV, D and L. Genotype D includes viruses isolated from all countries where MAYV has been detected, while genotype L strains have been found only in Brazil.2,3
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Chlorocebus aethiops kidney epithelial cells infected with MAYV, TRVL 4675.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-49913 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Chlorocebus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™)
Growth Medium: Eagle’s Minimum Essential Medium containing Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate and 1.5 g/L of sodium bicarbonate supplemented with 2% fetal bovine serum, or equivalent
Infection: Cells should be 75% to 90% confluent
Incubation: 1 to 5 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and detachment
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Tesh, R. B., Personal Communication.
2. Powers, A. M., et al. “Genetic Relationships among Mayaro and Una Viruses Suggest Distinct Patterns of Transmission.” Am. J. Trop. Med. Hyg. 75 (2006): 461- 469. PubMed: 16968922.
3. Auguste, A. J., et al. “Evolutionary and Ecological Characterization of Mayaro Virus Strains Isolated During an Outbreak, Venezuela, 2010.” Emerg. Infect. Dis. 21 (2015): 1742-1750. PubMed: 26401714.
4. LeDuc, J. W., F. Pinheiro, and A. Travassos da Rosa. “An Outbreak of Mayaro Virus Disease in Belterra, Brazil. II. Epidemiology.” Am. J. Trop. Med. Hyg. 30 (1981): 682- 688. PubMed: 6266264.
5. Long, K. C., et al. “Experimental Transmission of Mayaro Virus by Aedes aegypti.” Am. J. Trop. Med. Hyg. 85 (2011): 750-757. PubMed: 21976583.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH, as part of the WRCEVA program: Mayaro Virus, TRVL 4675, NR 49913."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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