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NR-50240   Zika virus, PRVABC59 (Human/2015/Puerto Rico)
(Viruses)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: Zika virus, PRVABC59 (Human/2015/Puerto Rico)
Organism: Zika virus
Biosafety Level: 2
Availability Status: In Stock
Store at: -60°C or colder
Contributor: BJ Russell
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Zika virus (ZIKV), PRVABC59 was isolated from the blood of a human in Puerto Rico in December 2015.

The complete genomic sequence of ZIKV, PRVABC59 has been determined (GenBank: KU501215). The complete coding sequence of NR-50240 lot 64112564 has also been determined (GenBank: KX087101). The E protein of the tissue culture grown ZIKV PRVABC59 isolate (GenBank: KX087101) has a valine to leucine mutation at position 330 relative to the original patient serum ZIKV PRVABC59 isolate (GenBank: KU501215). The ZIKV, PRVABC59 E-V330L mutant is shown to be less pathogenic in mice, with delayed mortality and decreased viral dissemination to eye and brain as compared to the original ZIKV PRVABC59 serum isolate.

Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells infected with ZIKV, PRVABC59.

Note: If homogeneity is required for your intended use, please purify prior to initiating work. Additional information and tools are available at ViPR (Virus Pathogen Resource).

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Zika Virus, PRVABC59, NR-50240."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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