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Product Name:
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Middle East Respiratory Syndrome Coronavirus, Florida/USA-2_Saudi Arabia_2014
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Nidovirales, Coronaviridae, Coronavirinae, Betacoronavirus
Agent: Middle East respiratory syndrome coronavirus (MERS-CoV)
Strain/Isolate: Florida/USA-2_Saudi Arabia_2014
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Chlorocebus aethiops kidney epithelial cells (Vero: ATCC® CCL-81™) infected with MERS-CoV, Florida/USA-2_Saudi Arabia_2014.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-50415 was packaged aseptically, in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Vero cells; ATCC® CCL-81™
Growth Medium: Dulbecco’s Modified Eagle’s Medium containing 4 mM L-glutamine, 1 mM sodium pyruvate, 4.5 g per L L-glucose and 1.5 g per L sodium bicarbonate, supplemented with 5% fetal bovine serum, or equivalent
Infection: Cells should be approximately 80% confluent
Incubation: 3 to 5 days at 37°C and 5% CO2
Cytopathic Effect: Refractile cell rounding, clumping, and detachment
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Bialek, S. R., et al. “First Confirmed Cases of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Infection in the United States, Updated Information on the Epidemiology of MERS-CoV Infection, and Guidance for the Public, Clinicians, and Public Health Authorities – May 2014.” MMWR Morb. Mortal. Wkly. Rep. 63 (2014): 431-436. PubMed: 24827411.
2. Tao, Y., et al. Division of Viral Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333, USA. 14 MAY 2014. Direct Submission.
3. Tao, Y., et al. Division of Viral Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, Georgia 30333, USA. 01 DEC 2014. Direct Submission.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Middle East Respiratory Syndrome Coronavirus, Florida/USA-2_Saudi Arabia_2014, NR-50415."
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Biosafety Level:
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3
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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