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Product Name:
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Cowden (Tissue Culture Adapted)
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Manufacturer:
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Linda J. Saif, Ph.D., Food Animal Health Research Program,
Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Wooster, Ohio, USA
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Taxonomy:
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Virus Classification: Caliciviridae, Sapovrius
Species: Porcine Sapovirus
Strain: Cowden
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Material Provided:
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Each vial contains approximately 1 mL of clarified cell lysate and supernatant from Sus scrofa kidney epithelial cells infected with tissue culture adapted PSV, Cowden.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-50552 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Sus scrofa kidney epithelial cells (LLC-PK, ATCC® CL-101™)
Growth Medium: Eagle’s Minimum Essential Medium with Earle's Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate, and 1500 mg/L sodium bicarbonate, 2% fetal bovine serum, 1% non-essential amino acids, and 50 µM bile acid (glycochenodeoxycholic acid).
Infection: Cells should be 80% to 90% confluent
Incubation: 4 to 5 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and detachment
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Saif, L. J., Personal Communication.
2. Flynn, T. F. and Saif, L. J. “Serial Propagation of Porcine Enteric Calicivirus-Like Virus in Primary Porcine Kidney Cell Cultures.” J. Clin. Microbiol. 26 (1988): 206-212. Pubmed: 2830305.
3. Parwani, A. V., et al. “Serial Propagation of Porcine Enteric Calicivirus in a Continuous Cell Line. Effect of Medium Supplementation with Intestinal Contents or Enzymes.” Arch. Virol. 120(1991): 115-122. PubMed: 1929875.
4. Guo, M., et al. “Molecular Characterization of a Porcine Enteric Calicivirus Genetically Related to Sapporo-Like Human Caliciviruses.” J. Virol. 73(1999): 9625-9631. PubMed: 10516074.
5. Scheuer, K. A., et al. “Prevalence of Porcine Noroviruses, Molecular Characterization of Emerging Porcine Sapoviruses from Finisher Swine in the United States, and Unified Classification Scheme for Sapoviruses.” J. Clin. Microbiol. 51 (2013): 2344-2353. PubMed: 23678065.
6. Chang, K-O., et al. “Bile Acids are Essential for Porcine Enteric Calicivirus Replication in Association with Down-Regulation of Signal Transducer and Activator of Transcription 1.” Proc. Natl. Acad. Sci. USA 101 (2004): 8733-8738. Pubmed: 15161971.
7. Lu, Z., et al. “Mechanism of Cell Culture Adaptation of an Enteric Calicivirus, the Porcine Sapovirus Cowden Strain.” J. Virol. 90 (2015): 1345-1358. Pubmed: 26581980.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Porcine Sapovirus, Cowden (Tissue Culture Adapted), NR-50552.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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