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Product Name:
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Lister (Elstree)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Poxviridae, Orthopoxvirus Agent: Vaccinia virus (VACV) Strain/Isolate: Lister (Elstree)
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Additional Information:
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Comments: VACV, strain Lister (Elstree) was deposited to ATCC® by Dr. James H. Nakano at the Centers for Disease Control in 1978. The Lister (Elstree) strain was widely used during the World Health Organization (WHO) program on the eradication of smallpox. 2,3 Although very effective, it induced rare but severe adverse effects. 4 This was one of the reasons for the discontinuation of vaccination after eradication. The complete genomic sequence of VACV, Lister has been determined (GenBank: : AY678276 ). 1 Vaccinia viruses are DNA viruses, belonging to the Poxviridae family that are closely related to smallpox-causing variola viruses. 5 Several strains of VACV were distributed as live vaccine during the global smallpox eradication program by WHO. Despite being an extremely well-studied virus, the origin of VACV is poorly understood. 5 VACV was first described in 1930s when it was demonstrated that the smallpox vaccination strain prevalent at that time was distinct from the earlier used cowpox virus. 6 During this time, it was assumed that VACV was laboratory attenuated and would not establish itself in nature. However, since then, several VACV strains have been described in zoonotic viral infections throughout the world. 6 |
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from African green monkey kidney (Vero) cells infected with VACV, Lister (Elstree).
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Packing/Storage:
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NR-51 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Vero cells (ATCC® CCL-81™) Growth Medium: Eagle’s Minimum Essential Medium supplemented with 2% fetal bovine serum, or equivalent (lot-specific details are on the Certificate of Analysis) Infection: Cells should be 80 to 90% confluent (not 100% confluent) Incubation: 6 to 8 days at 37°C and 5% CO2 Cytopathic Effect: Cell rounding and cell lysis
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Morikawa, S. et al. “An Attenuated LC16m8 Smallpox Vaccine: Analysis of Full-Genome Sequence and Induction of Immune Protection.” J. Virol. 79 (2005): 11873-11891. PubMed: 16140764. 2. Ober, B. T., et al. “Immunogenicity and Safety of Defective Vaccinia Virus Lister: Comparison with Modified Vaccinia Virus Ankara.” J. Virol. 76 (2002): 7713–7723. PubMed: 12097585. 3. Hsieh, S.-M., et al. “Clinical and Immunological Responses to Undiluted and Diluted Smallpox Vaccine with Vaccinia Virus of Lister Strain.” Vaccine 24 (2006): 510–515. PubMed: 16139395. 4. Auckland, C., A. Cowlishaw, D. Morgan, and E. Miller. “Reactions to Small Pox Vaccine in Naive and Previously-Vaccinated Individuals.” Vaccine 23 (2005): 4185–4187. PubMed: 15916840. 5. Sánchez-Sampedro, L., et al. “The Evolution of Poxvirus Vaccine.” Viruses 7 (2015): 1726-1803. PubMed: 25853483. 6. Oliveira, J. S., et al. “Vaccinia Virus Natural Infections in Brazil: The Good, the Bad, and the Ugly.” Viruses 9 (2017): E340. PubMed: 29140260. 7. Takahashi-Nishimaki, F., et al. “Genetic Analysis of Vaccinia Virus Lister Strain and Its Attenuated Mutant LC16m8: Production of Intermediate Variants by Homologous Recombination.” J. Gen. Virol. 68 (1987): 2705-2710. PubMed: 3668510.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus, Lister (Elstree), NR-51.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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