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Product Name:
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US/MO-KC/2014/001
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Picornaviridae, Parechovirus
Species: Parechovirus A type 3
Strain/Isolate: US/MO-KC/2014/001
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Additional Information:
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PeV-3 are single-stranded, positive-sense RNA viruses. PeVs were originally classified as enteroviruses but later assigned to a separate genus Parechovirus within family Picornaviridae.2,3,4 PeVs are increasingly detected worldwide as pathogens, particularly in infants and children, affecting the respiratory and gastrointestinal tract and the central nervous system.5,6,7 To date, 19 PeV genotypes, PeV-1 to PeV-19, have been identified based on phylogenetic analysis of viral protein 1 (VP1) sequences.4 PeV-1 and PeV-2 are the most commonly detected genotypes in respiratory and gastrointestinal infections whereas PeV-3 is most commonly recovered from cerebrospinal fluid.4,5,6,7
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Chlorocebus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™) infected with PeV-3, US/MO-KC/2014/001.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-51187 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Chlorocebus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™)
Growth Medium: Eagle’s Minimum Essential Medium containing Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate and 1.5 g/L of sodium bicarbonate supplemented with 2% fetal bovine serum, or equivalent
Infection: Cells should be 60% to 80% confluent
Incubation: 8 to 15 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and sloughing
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Selvarangan, R., Personal Communication.
2. Hyypiä, T., et al. “A Distinct Picornavirus Group Identified by Sequence Analysis.” Proc. Natl. Acad. Sci. USA 89 (1992): 8847-8851. PubMed: 1528901.
3. Stanway, G., et al. “Molecular and Biological Characteristics of Echovirus 22, a Representative of a New Picornavirus Group.” J. Virol. 68 (1994): 8232-8238. PubMed: 7966616.
4. Olijve, L., L. Jennings and T. Walls. “Human Parechovirus: An Increasingly Recognized Cause of Sepsis-Like Illness in Young Infants.” Clin. Microbiol. Rev. 31 (2017): e00047-17. PubMed: 29142080.
5. van der Sanden, S., et al. “Prevalence of Human Parechovirus in the Netherlands in 2000 to 2007.” J. Clin. Microbiol. 46 (2008): 2884–2889. PubMed: 18614653.
6. Selvarangan, R., et al. “Human Parechovirus 3 Causing Sepsis-Like Illness in Children from Midwestern United States.” Pediatr. Infect. Dis. 30 (2011): 238–242. PubMed: 20948454.
7. Sharp, J., et al. “Human Parechovirus in Respiratory Specimens from Children in Kansas City, Missouri.” J. Clin. Microbiol. 50 (2012): 4111-4113. PubMed: 23015672.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Parechovirus A Type 3, US/MO-KC/2014/001, NR-51187.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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