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Product Name:
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R119354b
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Ownership statement:
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This reagent is the property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Virus Classification: Flaviviridae, Orthoflavivirus, Orthoflavivirus louisense
Species: St. Louis Encephalitis Virus
Strain/Isolate: R119354b (also referred to as R119354)
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Additional Information:
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NR-51651 is a group B SLEV. A complete genome has not been sequenced. SLEV is a single-stranded RNA virus which is naturally maintained by mosquito to bird transmission involving Culex species mosquitos as vectors and passerine and columbiform avian species as amplificatory hosts.2,3,4 Small birds and a variety of mammals like bats, rodents, folivores and marsupials have also been shown to be infected.4 The virus has a wide distribution, encompassing tropical, sub-tropical and much of temperate-tropical zones of the Western Hemisphere, including most of North and South America.2 SLEV was first detected in North America in summer of 1933 in St. Louis, Missouri, where it resulted in 1,095 clinical human cases and 201 deaths.3 Most human SLEV infections are asymptomatic but clinical infections can range in severity from uncomplicated flu-like symptoms to fatal encephalitis; the incidence of severe disease and death increase with age.
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Material Provided:
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Each vial contains approximately 1 mL of cell lysate and supernatant from Chlorocebus aethiops kidney epithelial cells infected with SLEV, R119354b.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-51651 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Host: Chlorocebus aethiops kidney epithelial cells (Vero; ATCC® CCL-81™)
Growth Medium: Eagle’s Minimum Essential Medium containing Earle’s Balanced Salt Solution, non-essential amino acids, 2 mM L-glutamine, 1 mM sodium pyruvate and 1.5 g/L of sodium bicarbonate supplemented with 2% fetal bovine serum or equivalent
Infection: Cells should be 60% to 70% confluent
Incubation: 5 to 8 days at 37°C and 5% CO2
Cytopathic Effect: Cell rounding and sloughing
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Russell, B., Personal Communication.
2. Diaz, L. A., et al. “Exploring Genomic, Geographic and Virulence Interactions Among Epidemic and Non-Epidemic St. Louis Encephalitis Virus (Flavivirus) Strains.” PLoS One 10 (2015): e0136316. PubMed: 26312485.
3. Diaz, A., et al. “Reemergence of St. Louis Encephalitis in the Americas.” Emerg. Infect. Dis. 24 (2018): 2150-2157. PubMed: 30457961.
4. Kopp, A., et al. “Provenance and Geographic Spread of St. Louis Encephalitis Virus.” mBio 11 (2013): e00322. PubMed: 23760463.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: St. Louis Encephalitis Virus, R119354b, NR-51651.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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